Chronic exposure to arsenic, LINE-1 hypomethylation, and blood pressure: a cross-sectional study in Bangladesh

Environmental Health

Research

Keiko Nohara¹  Takehiro Suzuki¹  Khaled Hossain²  Atiqur Rahman²  Tanzina Tanu²  Farjana Nikkon²  M. M. Hasibuzzaman²  Shakhawoat Hossain²  Zahangir Alam Saud²  Md. Shofikul Islam³  Sudip Kumar Paul³  Mashiur Rahman⁴  Hideki Miyataka⁵  Seiichiro Himeno⁵ 

[1] Center for Health and Environmental Risk Research, National Institute for Environmental Studies, Tsukuba 305-8506, Japan;Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh;Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh;Department of Applied Nutrition and Food Technology, Islamic University, Kushtia-7003, Bangladesh;Exim Bank Agricultural University, Chapainawabganj, Bangladesh;Laboratory of Molecular Nutrition and Toxicology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8514, Japan;

关键词: Arsenic;    LINE-1;    Hypomethylation;    Blood pressure;    Hypertension;    Bangladesh;   

DOI  :  10.1186/s12940-017-0231-7

 received in 2016-10-06, accepted in 2017-03-01,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundChronic exposure to arsenic is associated with cancer and hypertension. Growing evidence suggests that altered methylation in long interspersed nuclear element-1 (LINE-1) is involved in many types of disorders, including cardiovascular disease. Here we evaluated the association between arsenic exposure and LINE-1 methylation levels, especially in relation to blood pressure (BP).MethodsA total of 236 subjects (175 from arsenic-endemic areas and 61 from a non-endemic area) in rural Bangladesh were recruited. The subjects’ arsenic exposure levels (i.e., drinking water, hair and nail arsenic concentrations) were measured by inductively coupled plasma mass spectroscopy. The subjects’ LINE-1 methylation levels were determined by pyrosequencing.ResultsThe average LINE-1 methylation levels of the subjects living in the arsenic-endemic areas were significantly (p < 0.01) lower than those of the subjects living in the non-endemic area. In a sex-stratified analysis, the arsenic exposure levels in female but not male subjects showed a significant inverse association with LINE-1 methylation levels before (water arsenic: p < 0.01, hair arsenic: p < 0.05, nail arsenic: p < 0.001) and after (water arsenic: p < 0.01, hair arsenic: p < 0.05, nail arsenic: p < 0.001) adjustment for age, body mass index and smoking. Analyses examining interactions among arsenic levels, BP and LINE-1 methylation showed that arsenic-related elevated levels of BP were associated with LINE-1 hypomethylation.ConclusionsOur findings demonstrated that chronic exposure to arsenic was inversely associated with LINE-1 methylation levels in blood leukocyte DNA and this was more pronounced in females than males; in addition, the decreased levels of LINE-1 methylation might be involved in the arsenic-induced elevation of BP.

【 授权许可】

CC BY   
© The Author(s). 2017