Comparison of the antibody responses to Plasmodium vivax and Plasmodium falciparum antigens in residents of Mandalay, Myanmar

Malaria Journal

Research

Youngjoo Sohn¹  Khin Lin²  Yoon Kong³  Byoung-Kuk Na⁴  Tong-Soo Kim⁵  Yeon-Joo Kim⁶  Myoung-Hee Kwon⁶  Jung-Yeon Kim⁶  Sung-Ung Moon⁶  Hyeong-Woo Lee⁷  Hyuck Kim⁸  Hyung-Hwan Kim⁹ 

[1] Department of Gynecology, College of Oriental Medicine, Sangji University, 220-717, Wonju, Republic of Korea;Department of Medical Research (Upper Myanmar), Pying Oo Lwin Township, Mandalay, Myanmar;Department of Molecular Parasitology, Center for Molecular Medicine, Samsung Biomedical Research Institute, School of Medicine, Sungkyunkwan University, 440-746, Suwon, Republic of Korea;Department of Parasitology and Institute of Health Sciences, Gyeongsang National University College of Medicine, 660-751, Jinju, Republic of Korea;Department of Parasitology, College of Medicine, Inha University, 405-751, Incheon, Republic of Korea;Division of Malaria and Parasitic Diseases, Korea Centers for Disease Control and Prevention, 363-951, Cheongwon-gun, Republic of Korea;Division of Malaria and Parasitic Diseases, Korea Centers for Disease Control and Prevention, 363-951, Cheongwon-gun, Republic of Korea;Division of Malaria and Parasitic Diseases, Korea Centers for Disease Control and Prevention, 363-951, Cheongwon-gun, Republic of Korea;Department of Pathology, University of Florida, J-566, 1600 SW Archer Road, 32610, Gainesville, FL, USA;International Research Center for Bioscience and Biotechnology, Jungwon University, 367-805, Goesan, Republic of Korea;Vascular Medicine Research Unit, Brigham and Women's Hospital, Harvard Medical School, 02139, Cambridge, MA, USA;International Research Center for Bioscience and Biotechnology, Jungwon University, 367-805, Goesan, Republic of Korea;

关键词: Malaria;    Patient Seron;    Indirect Fluorescent Antibody Test;    Smear Examination;    Thin Blood Smear;   

DOI  :  10.1186/1475-2875-10-228

 received in 2011-06-03, accepted in 2011-08-06,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe aim of this study was to investigate the profile of antibodies against several antigens of Plasmodium vivax and Plasmodium falciparum in Mandalay, Myanmar.MethodsMalaria parasites were identified by microscopic examination. To test the antibodies against P. vivax and P. falciparum in sera, an indirect immunofluorescence antibody test (IFAT) was performed using asexual blood parasite antigens. An enzyme-linked immunosorbent assay (ELISA) was performed with circumsporozoite protein (CSP), Pvs25 and Pvs28 recombinant proteins of transmission-blocking vaccine candidates for P. vivax, and liver stage specific antigen-1 and -3 (PfLSA-1, PfLSA-3) for P. falciparum.ResultsFourteen patients among 112 were found to be infected with P. vivax and 26 with P. falciparum by thick smear examination. Twenty-three patients were found to be infected with P. vivax, 19 with P. falciparum and five with both by thin smear examination. Blood samples were divided into two groups: Group I consisted of patients who were positive for infection by microscopic examination, and Group II consisted of those who showed symptoms, but were negative in microscopic examination. In P. falciparum, IgG against the blood stage antigen in Group I (80.8%) was higher than in Group II (70.0%). In P. vivax, IgG against the blood stage antigen in Group I (53.8%) was higher than in Group II (41.7%). However, the positivity rate of the PvCSP VK210 subtype in Group II (40.0%) was higher than in Group I (23.1%). Similarly for the PvCSP VK247 subtype, Group II (21.7%) was higher than that for Group I (9.6%). A similar pattern was observed in the ELISA using Pvs25 and Pvs28: positive rates of Group II were higher than those for Group I. However, those differences were not shown significant in statistics.ConclusionsThe positive rates for blood stage antigens of P. falciparum were higher in Group I than in Group II, but the positive rates for antigens of other stages (PfLSA-1 and -3) showed opposite results. Similar to P. falciparum, the positive rate of pre-blood stage (CSP VK210 and 247 subtype) and post-blood stage (Pvs25 and 28) antigens of P. vivax were higher in Group II than in Group I. Therefore, sero-diagnosis is not helpful to discriminate between malaria patients and symptomatic individuals during the epidemic season in Myanmar.

【 授权许可】

Unknown   
© Kim et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.