REAC technology as optimizer of stallion spermatozoa liquid storage

Reproductive Biology and Endocrinology

Research

Ignazio Cossu¹  Sabrina Caggiu²  Valeria Pasciu²  Fiammetta Berlinguer²  Sara Succu²  Daniela Addis²  Eraldo Sanna Passino²  Alessandro Castagna³  Salvatore Rinaldi⁴  Vania Fontani⁴ 

[1] AGRIS, Department of Research for Equine Reproduction, Ozieri, Sassari, Italy;Department of Veterinary Medicine, University of Sassari, Viale Vienna 43/B, 07100, Sassari, Italy;Departments of Regenerative Medicine, Rinaldi Fontani Institute, Viale Belfiore 43, 50144, Florence, Italy;Departments of Regenerative Medicine, Rinaldi Fontani Institute, Viale Belfiore 43, 50144, Florence, Italy;Research Department, Rinaldi Fontani Foundation, Viale Belfiore 43, 50144, Florence, Italy;

关键词: Comet Assay;    Sperm Cell;    Sperm Motility;    Trolox Equivalent Antioxidant Capacity;    Liquid Storage;   

DOI  :  10.1186/s12958-017-0229-6

 received in 2016-11-30, accepted in 2017-02-03,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundREAC technology (acronym for Radio Electric Asymmetric Conveyor) is a technology platform for neuro and bio modulation. It has already proven to optimize the ions fluxes at the molecular level and the molecular mechanisms driving cellular asymmetry and polarization.MethodsThis study was designed to verify whether this technology could extend spermatozoa life-span during liquid storage, while preserving their functions, DNA integrity and oxidative status. At 0, 24, 48, and 72 h. of storage at 4 °C, a battery of analyses was performed to assess spermatozoa viability, motility parameters, acrosome status, and DNA integrity during REAC treatment. Spermatozoa oxidative status was assessed by determining lipid peroxidation, the activity of superoxide dismutase (SOD), and the total antioxidant capacity.ResultsDuring liquid storage REAC treated spermatozoa, while not showing an increased viability nor motility compared to untreated ones, had a higher acrosome (p > 0.001) and DNA integrity (p > 0.01). Moreover, the analysis of the oxidative status indicated that the mean activity of the intracellular superoxide dismutase (SOD) was significantly higher in REAC treated spermatozoa compared to untreated controls (p < 0.05), while the intracellular concentration of malondialdehyde (MDA), an end product of lipid peroxidation, at the end of the REAC treatment was higher in untreated controls (p > 0.05). The REAC efficacy on spermatozoa oxidative status was also evidenced by the higher trolox equivalent antioxidant capacity (TEAC) found in both the cellular extract (p < 0.05) and the storage media of REAC treated spermatozoa compared to untreated controls (p < 0.0001).ConclusionThe present study demonstrated that REAC treatment during liquid storage preserves spermatozoa acrosome membrane and DNA integrity, likely due to the enhancement of sperm antioxidant defenses. These results open new perspective about the extending of spermatozoa functions in vitro and the clinical management of male infertility.

【 授权许可】

CC BY   
© The Author(s). 2017