Deficiency of maize starch-branching enzyme i results in altered starch fine structure, decreased digestibility and reduced coleoptile growth during germination

BMC Plant Biology

Research Article

Marna Yandeau-Nelson¹  Donald B Thompson²  Mark J Guiltinan³  Huan Xia⁴ 

[1] Department of Biochemistry, Biophysics & Molecular Biology, Iowa State University, 50011-3260, Ames, Iowa, USA;Department of Horticulture, The Pennsylvania State University, 16802-5807, University Park, Pennsylvania, USA;Department of Food Science, The Pennsylvania State University, 16802-2504, University Park, Pennsylvania, USA;Department of Horticulture, The Pennsylvania State University, 16802-5807, University Park, Pennsylvania, USA;MARS Petcare US, 315 Cool Springs Boulevard, 37067, Franklin, Tennessee, USA;Department of Food Science, The Pennsylvania State University, 16802-2504, University Park, Pennsylvania, USA;

关键词: Starch;    Amylose;    Starch Granule;    Native Starch;    Starch Biosynthesis;   

DOI  :  10.1186/1471-2229-11-95

 received in 2010-12-10, accepted in 2011-05-21,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundTwo distinct starch branching enzyme (SBE) isoforms predate the divergence of monocots and dicots and have been conserved in plants since then. This strongly suggests that both SBEI and SBEII provide unique selective advantages to plants. However, no phenotype for the SBEI mutation, sbe1a, had been previously observed. To explore this incongruity the objective of the present work was to characterize functional and molecular phenotypes of both sbe1a and wild-type (Wt) in the W64A maize inbred line.ResultsEndosperm starch granules from the sbe1a mutant were more resistant to digestion by pancreatic α-amylase, and the sbe1a mutant starch had an altered branching pattern for amylopectin and amylose. When kernels were germinated, the sbe1a mutant was associated with shorter coleoptile length and higher residual starch content, suggesting that less efficient starch utilization may have impaired growth during germination.ConclusionsThe present report documents for the first time a molecular phenotype due to the absence of SBEI, and suggests strongly that it is associated with altered physiological function of the starch in vivo. We believe that these results provide a plausible rationale for the conservation of SBEI in plants in both monocots and dicots, as greater seedling vigor would provide an important survival advantage when resources are limited.

【 授权许可】

Unknown   
© Xia et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.