Tissue specific analysis reveals a differential organization and regulation of both ethylene biosynthesis and E8 during climacteric ripening of tomato

BMC Plant Biology

Research Article

Rita Derua¹  Etienne Waelkens¹  Sandy Vandoninck¹  Jos Vanderleyden²  Stijn Spaepen²  Toon Nicolay²  Maurice P De Proft³  Nick Vandenzavel⁴  Inge Bulens⁴  Annemie H Geeraerd⁴  Maarten LATM Hertog⁴  Ifigeneia Mellidou⁴  Bram Van de Poel⁵  Cindy Smet⁶  Bart M Nicolai⁷ 

[1] Department of Cellular and Molecular Medicine, KU Leuven, Herestraat 49, 3000, Leuven, Belgium;Department of Microbial and Molecular Systems, Center of Microbial and Plant Genetics, KU Leuven, Kasteelpark Arenberg 20, bus 2460, 3001, Leuven, Belgium;Division of Crop Biotechnics, Department of Biosystems, KU Leuven, Willem de Croylaan 42, 3001, Leuven, Belgium;Division of Mechatronics, Biostatistics and Sensors (MeBioS), Department of Biosystems (BIOSYST), KU Leuven, Willem de Croylaan 42, bus 2428, 3001, Leuven, Belgium;Division of Mechatronics, Biostatistics and Sensors (MeBioS), Department of Biosystems (BIOSYST), KU Leuven, Willem de Croylaan 42, bus 2428, 3001, Leuven, Belgium;Department of Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Bldg 413, 20742, College Park, MD, USA;Division of Mechatronics, Biostatistics and Sensors (MeBioS), Department of Biosystems (BIOSYST), KU Leuven, Willem de Croylaan 42, bus 2428, 3001, Leuven, Belgium;Division of Chemical and Biochemical Process Technology and Control Section, Department of Chemical Engineering, KU Leuven, Willem de Croylaan 46, 3001, Leuven, Belgium;Division of Mechatronics, Biostatistics and Sensors (MeBioS), Department of Biosystems (BIOSYST), KU Leuven, Willem de Croylaan 42, bus 2428, 3001, Leuven, Belgium;Flanders Centre of Postharvest Technology (VCBT), Willem de Croylaan 42, 3001, Leuven, Belgium;

关键词: Solanum lycopersicum;    Tomato;    Ethylene biosynthesis;    Tissues;    Pericarp;    Septa;    Columella;    Placenta;    Seeds;    Locular gel;    E8;   

DOI  :  10.1186/1471-2229-14-11

 received in 2013-07-18, accepted in 2014-01-04,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundSolanum lycopersicum or tomato is extensively studied with respect to the ethylene metabolism during climacteric ripening, focusing almost exclusively on fruit pericarp. In this work the ethylene biosynthesis pathway was examined in all major tomato fruit tissues: pericarp, septa, columella, placenta, locular gel and seeds. The tissue specific ethylene production rate was measured throughout fruit development, climacteric ripening and postharvest storage. All ethylene intermediate metabolites (1-aminocyclopropane-1-carboxylic acid (ACC), malonyl-ACC (MACC) and S-adenosyl-L-methionine (SAM)) and enzyme activities (ACC-oxidase (ACO) and ACC-synthase (ACS)) were assessed.ResultsAll tissues showed a similar climacteric pattern in ethylene productions, but with a different amplitude. Profound differences were found between tissue types at the metabolic and enzymatic level. The pericarp tissue produced the highest amount of ethylene, but showed only a low ACC content and limited ACS activity, while the locular gel accumulated a lot of ACC, MACC and SAM and showed only limited ACO and ACS activity. Central tissues (septa, columella and placenta) showed a strong accumulation of ACC and MACC. These differences indicate that the ethylene biosynthesis pathway is organized and regulated in a tissue specific way. The possible role of inter- and intra-tissue transport is discussed to explain these discrepancies. Furthermore, the antagonistic relation between ACO and E8, an ethylene biosynthesis inhibiting protein, was shown to be tissue specific and developmentally regulated. In addition, ethylene inhibition by E8 is not achieved by a direct interaction between ACO and E8, as previously suggested in literature.ConclusionsThe Ethylene biosynthesis pathway and E8 show a tissue specific and developmental differentiation throughout tomato fruit development and ripening.

【 授权许可】

Unknown   
© Van de Poel et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.