K‐252a inhibits the response of tomato cells to fungal elicitors in vivo and their microsomal protein kinase in vitro

FEBS Letters

Felix, Georg¹  Grosskopf, Debora G.¹  Boller, Thomas¹ 

[1] Friedrich Miescher-Institute, Basel, Switzerland

关键词: Ethylene biosynthesis;    Phenylalanine ammonia-lyase;    K-252a;    Protein kinase;    Elicitor;    Tomato;   

DOI  :  10.1016/0014-5793(90)81466-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two characteristic responses of plant cells to fungal elicitors, induction of phenylalanine ammonia-lyase activity and of ethylene biosynthesis, were studied in suspension-cultured tomato cells. Induction of both responses was completely blocked by 500 nM K-252a, a known inhibitor of mammalian protein kinases. About 100 nM K-252a caused half-maximal inhibition. In vitro, K-252a inhibited protein kinase activity in microsomal preparations from tomato cells. Inhibition was competitive with respect to ATP and had a K ₁ of about 15 nM. Thus, protein kinases sensitive to K-252a occur in plants and might be important for the plant's response to fungal elicitors.

【 授权许可】

Unknown