Hyperploidization of V79 Cells by K-252a in Comparison with Demecolcine

Cell Structure and Function

Kohzaburo Fujikawa-Yamamoto¹  Shizuo Odashima¹  Kouhei Teraoka¹ 

[1]Division of Basic Science, Research Institute of Medical Science, Kanazawa Medical University

关键词: K-252a;    demecolcine;    flow cytometry;    cell cycle;    hyperploidization;   

DOI  :  10.1247/csf.18.449

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(11)Cited-By(10)The cellular responses of the V79 cell cycle to K-252a, a protein kinase inhibitor, were examined by flow cytometry (FCM) and by time-lapse videomicrography. At a concentration of 0.5 μM of K-252a for 72 h, V79 cells became hyperploid, having above 32c DNA content. The cycle times in the hyperploidizing process were 11.2 (4c-8c), 16.3 (8c-16c) and 19.3 (16c-32c) hours, values which were less than twice that of the control (10.5 h). After the washout of K-252a, the DNA content was widely distributed in the V79 cell population and hyperploid V79 cells reduced DNA content through a variety of cell division modes. These cellular responses to hyperploidization of V79 cells by K-252a were in reasonable agreement with those by demecolcine.

【 授权许可】

Unknown