Exposure to ambient particulate matter alters the microbial composition and induces immune changes in rat lung

Respiratory Research

Research

Pixin Ran¹  Baoling Liao¹  Yuming Zhou¹  Naijian Li¹  Bing Li²  Fang He³ 

[1] State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang Road, 510120, Guangzhou, China;The GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, China;The School of Basic Medicine, Guangzhou Medical University, Guangzhou, China;

关键词: Particulate matter;    Biomass fuel;    Motor vehicle exhaust;    Microbial composition;    Immune response;   

DOI  :  10.1186/s12931-017-0626-6

 received in 2017-04-25, accepted in 2017-07-19,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundAmbient particulate matter exposure has been shown to increase the risks of respiratory diseases. However, the role of the lung microbiome and the immune response to inhaled particulate matter are largely unexplored. We studied the influence of biomass fuel and motor vehicle exhaust particles on the lung microbiome and pulmonary immunologic homeostasis in rats.MethodsFifty-seven Sprague–Dawley rats were randomly divided into clean air (CON), biomass fuel (BMF), and motor vehicle exhaust (MVE) groups. After a 4-week exposure, the microbial composition of the lung was assessed by 16S rRNA pyrosequencing, the structure of the lung tissue was assessed with histological analysis, the phagocytic response of alveolar macrophages to bacteria was determined by flow cytometry, and immunoglobulin concentrations were measured with commercial ELISA kits.ResultsThere was no significant difference in lung morphology between the groups. However, the BMF and MVE groups displayed greater bacterial abundance and diversity. Proteobacteria were present in higher proportions in the MVE group, and 12 bacterial families differed in their relative abundances between the three groups. In addition, particulate matter exposure significantly increased the capacity of alveolar macrophages to phagocytose bacteria and induced changes in immunoglobulin levels.ConclusionWe demonstrated that particulate matter exposure can alter the microbial composition and change the pulmonary immunologic homeostasis in the rat lung.

【 授权许可】

CC BY   
© The Author(s). 2017