Chronic exposure to ambient particulate matter induces gut microbial dysbiosis in a rat COPD model

Respiratory Research

Naijian Li¹  Zhaowei Yang¹  Jinding Pu²  Baoling Liao³  Binwei Hao⁴  Zhenli Fu⁴  Weitao Cao⁴  Pixin Ran⁴  Tianhui Pan⁴  Yuming Zhou⁴  Bing Li⁵  Fang He⁶ 

[1] Department of Allergy and Clinical Immunology, State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, 510000, Guangzhou, P.R. China;Department of Respiratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University, 510000, Guangzhou, P.R. China;Nanhai Hospital Southern Medical University, 528000, Foshan, P.R. China;State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, 510000, Guangzhou, P.R. China;The GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, 510000, Guangzhou, P.R. China;The School of Basic Medicine, Guangzhou Medical University, 510000, Guangzhou, P.R. China;

关键词: Biomass fuel;    Motor vehicle exhaust;    COPD;    Gut microbiome;   

DOI  :  10.1186/s12931-020-01529-3

来源: Springer

【 摘 要 】

BackgroundThe role of the microbiota in the pathogenesis of chronic obstructive pulmonary disease (COPD) following exposure to ambient particulate matter (PM) is largely unknown.MethodsFifty-four male Sprague-Dawley rats were exposed to clean air, biomass fuel (BMF), or motor vehicle exhaust (MVE) for 4, 12, and 24 weeks. We performed pulmonary inflammation evaluation, morphometric measurements, and lung function analysis in rat lung at three different times points during exposure. Lung and gut microbial composition was assessed by 16S rRNA pyrosequencing. Serum lipopolysaccharide levels were measured and short-chain fatty acids in colon contents were quantified.ResultsAfter a 24-week PM exposure, rats exhibited pulmonary inflammation and pathological changes characteristic of COPD. The control and PM exposure (BMF and MVE) groups showed similar microbial diversity and composition in rat lung. However, the gut microbiota after 24 weeks PM exposure was characterized by decreased microbial richness and diversity, distinct overall microbial composition, lower levels of short-chain fatty acids, and higher serum lipopolysaccharide.ConclusionChronic exposure to ambient particulate matter induces gut microbial dysbiosis and metabolite shifts in a rat model of chronic obstructive pulmonary disease.

【 授权许可】

CC BY