Characterization of flagellins isolated from a highly motile strain of Lactobacillus agilis

BMC Microbiology

Research Article

Tomohiro Irisawa¹  Sanae Okada²  Emiko Midorikawa²  Kazuho Kondo²  Akinobu Kajikawa²  Kazuya Masuda³  Shizunobu Igimi³ 

[1] Department of Animal Science, Tokyo University of Agriculture, 1737 Funako, 243-0034, Atsugi, Kanagawa, Japan;Department of Applied Biology and Chemistry, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, 156-8502, Setagaya, Tokyo, Japan;Division of Biomedical Food Research, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya, Tokyo, Japan;

关键词: Lactobacillus;    Lactic Acid Bacterium;    Immunological Activity;    Early Exponential Phase;    Flagellin Gene;   

DOI  :  10.1186/s12866-016-0667-x

 received in 2015-09-07, accepted in 2016-03-07,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMost lactic acid bacteria are non-motile but some of them are flagellated and exhibit motility. So far, motile lactobacilli have rarely been studied, and characteristics of their flagellins are poorly understood. In this study, a highly motile strain of Lactobacillus agilis was recruited for transcriptional analysis and characterization of its flagellins.ResultsUnlike another motile lactic acid bacteria of intestinal isolate, Lactobacillus ruminis, flagellar filaments of the L. agilis strain probably consist of two homologous but distinct flagellins. Glycosylation of the flagellar filaments and their resistance to heat, acid and SDS were also observed. The immunological activity of the flagellins was evaluated through the stimulation of Caco-2 cells. The results show that TLR5-stimulating activity of the protein is attenuated, likely due to an incomplete TLR5-recognition site.ConclusionsThe flagella filaments of L. agilis BKN88 consist of two homologous glycosylated flagellins, which likely have an incomplete TLR5-recognition site. The characteristics of the flagellin are presumably a consequence of adaptation as a commensal microbe in the gastrointestinal tract.

【 授权许可】

CC BY   
© Kajikawa et al. 2016