Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs)

Proteome Science

Review

Md. Mamunul Haque¹  Dhiraj P. Murale¹  Seong Cheol Hong²  Jun-Seok Lee² 

[1] Molecular Recognition Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, 136-791, Seoul, Republic of Korea;Molecular Recognition Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, 136-791, Seoul, Republic of Korea;Department of Biological Chemistry, KIST-School UST, 39-1 Hawolgok-dong, 136-791, Seoul, Republic of Korea;

关键词: Photo-affinity probe;    Protein-protein interaction;    Quantitative proteomics;    Benzophenone;    Aryl azide;    Diazirine;   

DOI  :  10.1186/s12953-017-0123-3

 received in 2017-04-18, accepted in 2017-06-15,  发布年份 2017

来源: Springer

【 摘 要 】

Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.

【 授权许可】

CC BY   
© The Author(s). 2017