Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs)

Proteome Science

卷:15

Md. Mamunul Haque¹  Jun-Seok Lee¹  Dhiraj P. Murale¹  Seong Cheol Hong¹ 

[1] Molecular Recognition Research Center, Korea Institute of Science and Technology;

关键词: Photo-affinity probe;    Protein-protein interaction;    Quantitative proteomics;    Benzophenone;    Aryl azide;    Diazirine;   

DOI  :  10.1186/s12953-017-0123-3

来源: DOAJ

【 摘 要 】

Abstract Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.

【 授权许可】

Unknown