Characterization of proteome alterations in Phanerochaete chrysosporium in response to lead exposure

Proteome Science

Research

Gülay Özcengiz¹  Volkan Yıldırım¹  Servet Özcan²  Dörte Becher³  Michael Hecker³  Knut Büttner³ 

[1] Department of Biological Sciences, Middle East Technical University, Ankara, Turkey;Department of Biology, Erciyes University, Kayseri, Turkey;Institut für Mikrobiologie, Ernst-Moritz Arndt-Universität Greifswald, Greifswald, Germany;

关键词: Lead Exposure;    mRNA Splice Factor;    Core Environmental Stress Response;    Sabaroud Dextrose Agar;    Chrysosporium Cell;   

DOI  :  10.1186/1477-5956-9-12

 received in 2010-08-13, accepted in 2011-03-09,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundTotal soluble proteome alterations of white rot fungus Phanerochaete chrysosporium in response to different doses (25, 50 and 100 μM) of Pb (II) were characterized by 2DE in combination with MALDI-TOF-MS.ResultsDose-dependent molecular response to Pb (II) involved a total of 14 up-regulated and 21 down-regulated proteins. The induction of an isoform of glyceraldehyde 3-phosphate dehydrogenase, alcohol dehydrogenase class V, mRNA splicing factor, ATP-dependent RNA helicase, thioredoxin reductase and actin required a Pb (II) dose of at least 50 μM. Analysis of the proteome dynamics of mid-exponential phase cells of P. chrysosporium subjected to 50 μM lead at exposure time intervals of 1, 2, 4 and 8 h, identified a total of 23 proteins in increased and 67 proteins in decreased amount. Overall, the newly induced/strongly up-regulated proteins involved in (i) amelioration of lipid peroxidation products, (ii) defense against oxidative damage and redox metabolism, (iii) transcription, recombination and DNA repair (iv) a yet unknown function represented by a putative protein.ConclusionThe present study implicated the particular role of the elements of DNA repair, post-tanscriptional regulation and heterotrimeric G protein signaling in response to Pb (II) stress as shown for the first time for a basidiomycete.

【 授权许可】

Unknown   
© Yıldırım et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.