Small RNA interference-mediated gene silencing of heparanase abolishes the invasion, metastasis and angiogenesis of gastric cancer cells

BMC Cancer

Research Article

Jihua Dong¹  Xiaohua Hou²  Liduan Zheng³  Qiangsong Tong⁴  Hong Mei⁴  Guosong Jiang⁴  Jiarui Pu⁴ 

[1] Department of Central Laboratory, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, China;Department of Gastroenterology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, China;Department of Pathology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, China;Department of Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei Province, China;

关键词: Gastric Cancer;    Gastric Cancer Cell;    Advanced Gastric Cancer;    Human Gastric Cancer Cell;    Cell Adhesion Assay;   

DOI  :  10.1186/1471-2407-10-33

 received in 2009-08-03, accepted in 2010-02-05,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundHeparanase facilitates the invasion and metastasis of cancer cells, and is over-expressed in many kinds of malignancies. Our studies indicated that heparanase was frequently expressed in advanced gastric cancers. The aim of this study is to determine whether silencing of heparanase expression can abolish the malignant characteristics of gastric cancer cells.MethodsThree heparanase-specific small interfering RNA (siRNAs) were designed, synthesized, and transfected into cultured gastric cancer cell line SGC-7901. Heparanase expression was measured by RT-PCR, real-time quantitative PCR and Western blot. Cell proliferation was detected by MTT colorimetry and colony formation assay. The in vitro invasion and metastasis of cancer cells were measured by cell adhesion assay, scratch assay and matrigel invasion assay. The angiogenesis capabilities of cancer cells were measured by tube formation of endothelial cells.ResultsTransfection of siRNA against 1496-1514 bp of encoding regions resulted in reduced expression of heparanase, which started at 24 hrs and lasted for 120 hrs post-transfection. The siRNA-mediated silencing of heparanase suppressed the cellular proliferation of SGC-7901 cells. In addition, the in vitro invasion and metastasis of cancer cells were attenuated after knock-down of heparanase. Moreover, transfection of heparanase-specific siRNA attenuated the in vitro angiogenesis of cancer cells in a dose-dependent manner.ConclusionsThese results demonstrated that gene silencing of heparanase can efficiently abolish the proliferation, invasion, metastasis and angiogenesis of human gastric cancer cells in vitro, suggesting that heparanase-specific siRNA is of potential values as a novel therapeutic agent for human gastric cancer.

【 授权许可】

Unknown   
© Zheng et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.