Excretory/secretory products from Trichinella spiralis adult worms ameliorate myocardial infarction by inducing M2 macrophage polarization in a mouse model

Parasites & Vectors

Research

Jutai Wen¹  Shuyao Duan¹  Qiuyu Zhu¹  Huihui Li²  Xiaoli Wang²  Xiaodi Yang²  Shuying Wang³  Weixiao Zhang³  Wenhui Yin³  Lingqin Wu⁴  Shili Wu⁵  Rui Zhou⁵  Erhe Gao⁶  Bin Zhan⁷ 

[1] Anhui Key Laboratory of Infection and Immunity of Bengbu Medical College, 233000, Bengbu, China;Anhui Key Laboratory of Infection and Immunity of Bengbu Medical College, 233000, Bengbu, China;Basic Medical College of Bengbu Medical College, 233000, Bengbu, China;Anhui Key Laboratory of Infection and Immunity of Bengbu Medical College, 233000, Bengbu, China;First Affiliated Hospital of Bengbu Medical College, 233000, Bengbu, China;Anhui Key Laboratory of Infection and Immunity of Bengbu Medical College, 233000, Bengbu, China;First Affiliated Hospital of Bengbu Medical College, 233000, Bengbu, China;Second Affiliated Hospital of Jiaxing University, 314000, Jiaxing, China;First Affiliated Hospital of Bengbu Medical College, 233000, Bengbu, China;Lewis Katz School of Medicine, Temple University, 19140, Philadelphia, PA, USA;National School of Tropical Medicine, Baylor College of Medicine, 77030, Houston, TX, USA;

关键词: Myocardial infarction;    Excretory-secretory products;    Trichinella spiralis;    Macrophage;    Immunomodulation;   

DOI  :  10.1186/s13071-023-05930-x

 received in 2023-05-26, accepted in 2023-08-14,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundIschemia-induced inflammatory response is the main pathological mechanism of myocardial infarction (MI)-caused heart tissue injury. It has been known that helminths and worm-derived proteins are capable of modulating host immune response to suppress excessive inflammation as a survival strategy. Excretory/secretory products from Trichinella spiralis adult worms (Ts-AES) have been shown to ameliorate inflammation-related diseases. In this study, Ts-AES were used to treat mice with MI to determine its therapeutic effect on reducing MI-induced heart inflammation and the immunological mechanism involved in the treatment.MethodsThe MI model was established by the ligation of the left anterior descending coronary artery, followed by the treatment of Ts-AES by intraperitoneal injection. The therapeutic effect of Ts-AES on MI was evaluated by measuring the heart/body weight ratio, cardiac systolic and diastolic functions, histopathological change in affected heart tissue and observing the 28-day survival rate. The effect of Ts-AES on mouse macrophage polarization was determined by stimulating mouse bone marrow macrophages in vitro with Ts-AES, and the macrophage phenotype was determined by flow cytometry. The protective effect of Ts-AES-regulated macrophage polarization on hypoxic cardiomyocytes was determined by in vitro co-culturing Ts-AES-induced mouse bone marrow macrophages with hypoxic cardiomyocytes and cardiomyocyte apoptosis determined by flow cytometry.ResultsWe observed that treatment with Ts-AES significantly improved cardiac function and ventricular remodeling, reduced pathological damage and mortality in mice with MI, associated with decreased pro-inflammatory cytokine levels, increased regulatory cytokine expression and promoted macrophage polarization from M1 to M2 type in MI mice. Ts-AES-induced M2 macrophage polarization also reduced apoptosis of hypoxic cardiomyocytes in vitro.ConclusionsOur results demonstrate that Ts-AES ameliorates MI in mice by promoting the polarization of macrophages toward the M2 type. Ts-AES is a potential pharmaceutical agent for the treatment of MI and other inflammation-related diseases.Graphical Abstract

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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