Identifying the molecular and cellular signature of cardiac dilation following myocardial infarction

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1865

Article

Lindsey, Merry L.^1,2,3  Ma, Yonggang¹  Flynn, Elizabeth R.¹  Winniford, Michael D.^1,3  Hall, Michael E.^1,3  DeLeon-Pennell, Kristine Y.^4,5 

[1] Univ Mississippi, Med Ctr, Dept Physiol & Biophys, Mississippi Ctr Heart Res, 2500 N State St, Jackson, MS 39216 USA

[2] GV Sonny Montgomery Vet Affairs Med Ctr, Res Serv, 1500 E Woodrow Wilson Ave, Jackson, MS 39216 USA

[3] Univ Mississippi, Med Ctr, Div Cardiol, 2500 N State St, Jackson, MS 39216 USA

[4] Ralph H Johnson Vet Affairs Med Ctr, Res Serv, 109 Bee St, Charleston, SC 29401 USA

[5] Med Univ South Carolina, Div Cardiol, 30 Courtenay Dr, Charleston, SC 29425 USA

关键词: Big data;    Cardiovascular disease;    Myocardial infarction;    LV dilation;    Inflammation;    Macrophage;    Proteomics;   

DOI  :  10.1016/j.bbadis.2018.09.023

来源: Elsevier

【 摘 要 】

Establishing molecular and cellular indicators that reflect the extent of dilation of the left ventricle (LV) after myocardial infarction (MI) may improve diagnostic and prognostic capabilities. We queried the Mouse Heart Attack Research Tool (mHART) 1.0 for day 7 post-MI mice (age 3-9 months, untreated males and females) with serial echocardiographic data at days 0, 1, and 7 (n = 51). Mice were classified into two subgroups determined by a median fold change of 1.6 in end-diastolic dimensions (EDD) normalized to pre-MI values; n = 26 fell below (moderate; mean of 1.42 +/- 0.01) and n = 25 fell above this cut-off (extreme; mean of 1.79 +/- 0.01; p < 0.001 vs. moderate). Plasma proteomic profiling of 34 analytes measured at day 7 post-MI from male mice (n = 12 moderate and 12 extreme) were evaluated as the test dataset, and receiver operating curve (ROC) analysis was used to assess strength of biomarkers. Females (n = 6 moderate and 9 extreme) were used as the validation dataset. Both by t-test and characteristic (ROC) curve analysis, lower macrophage inflammatory protein-1 gamma (MIP-1 gamma), lymphotactin, and granulocyte chemotactic protein-2 (GCP-2) were identified as plasma indicators for dilation status (p < 0.05 for all). Macrophage numbers were decreased and complement C5, laminin 1, and Ccr8 gene levels were significantly higher in the LV infarcts of the extreme dilation group (p < 0.05 for all). A composite panel including plasma MIP-1 gamma, lymphotactin, and GCP-2, and LV infarct Ccr8 and macrophage numbers strongly mirrored LV dilation status (AUC = 0.92; p < 0.0001). Using the mHART 1.0 database, we determined that a failure to mount sufficient macrophage-mediated inflammation was indicative of exacerbated LV dilation.

【 授权许可】

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