Citrus flavonoids repress the mRNA for stearoyl-CoA desaturase, a key enzyme in lipid synthesis and obesity control, in rat primary hepatocytes

Lipids in Health and Disease

Short Report

Shivendra D Shukla¹  LaNita A Nichols¹  Lené J Holland¹  Daniel E Jackson¹  John A Manthey² 

[1] Department of Medical Pharmacology and Physiology, University of Missouri School of Medicine, 65212, Columbia, Missouri, USA;United States Department of Agriculture, Agricultural Research Service, Citrus and Subtropical Products Laboratory, 33881, Winter Haven, Florida, USA;

关键词: Flavonoid;    HepG2 Cell;    Naringenin;    Hesperetin;    Sterol Regulatory Element Binding Protein;   

DOI  :  10.1186/1476-511X-10-36

 received in 2010-12-13, accepted in 2011-02-23,  发布年份 2011

来源: Springer

【 摘 要 】

Citrus flavonoids have been shown to decrease plasma lipid levels, improve glucose tolerance, and attenuate obesity. One possible mechanism underlying these physiological effects is reduction of hepatic levels of the mRNA for stearoyl-CoA desaturase-1 (SCD1), since repression of this enzyme reduces hyperlipidemia and adiposity. Here, we show that citrus flavonoids of two structural classes reduce SCD1 mRNA concentrations in a dose-dependent manner in rat primary hepatocytes. This is the first demonstration of repression of SCD1 by citrus flavonoids, either in vivo or in cultured cells. Furthermore, it is the first use of freshly-isolated hepatocytes from any animal to examine citrus flavonoid action at the mRNA level. This study demonstrates that regulation of SCD1 gene expression may play a role in control of obesity by citrus flavonoids and that rat primary hepatocytes are a physiologically-relevant model system for analyzing the molecular mechanisms of flavonoid action in the liver.

【 授权许可】

Unknown   
© Nichols et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.