Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen

Journal of Nanobiotechnology

Research

Hyun Ah Cho¹  Young Keun Kim¹  Chan-Ki Min²  Hong-il Kim²  Nguyen Thi Hai Yen²  Na-Young Ha²  Prashant Sharma²  Hyun Mu Shin³  Nam-Hyuk Cho³  Ik-Sang Kim⁴  Myung-Sik Choi⁴  Jae-Seung Kang⁵ 

[1] Department of Materials Science and Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, 02841, Seoul, Republic of Korea;Department of Microbiology and Immunology, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-gu, 03080, Seoul, Republic of Korea;Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea;Department of Microbiology and Immunology, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-gu, 03080, Seoul, Republic of Korea;Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea;Institute of Endemic Disease, Seoul National University Medical Research Center and Bundang Hospital, Seoul, Republic of Korea;Department of Microbiology and Immunology, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-gu, 03080, Seoul, Republic of Korea;Institute of Endemic Disease, Seoul National University Medical Research Center and Bundang Hospital, Seoul, Republic of Korea;Department of Microbiology, Inha University School of Medicine, Incheon, Republic of Korea;

关键词: Zinc oxide nanoparticle;    ZnO binding peptide;    Scrub typhus;    Vaccine;   

DOI  :  10.1186/s12951-016-0229-2

 received in 2016-09-06, accepted in 2016-11-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundZinc oxide nanoparticle (ZNP) has been applied in various biomedical fields. Here, we investigated the usage of ZNP as an antigen carrier for vaccine development by combining a high affinity peptide to ZNP.ResultsA novel zinc oxide-binding peptide (ZBP), FPYPGGDA, with high affinity to ZNP (Ka = 2.26 × 106 M−1) was isolated from a random peptide library and fused with a bacterial antigen, ScaA of Orientia tsutsugamushi, the causative agent of scrub typhus. The ZNP/ZBP-ScaA complex was efficiently phagocytosed by a dendritic cell line, DC2.4, in vitro and significantly enhanced anti-ScaA antibody responses in vivo compared to control groups. In addition, immunization with the ZNP/ZBP-ScaA complex promoted the generation of IFN-γ-secreting T cells in an antigen-dependent manner. Finally, we observed that ZNP/ZBP-ScaA immunization provided protective immunity against lethal challenge of O. tsutsugamushi, indicating that ZNP can be used as a potent adjuvant when complexed with ZBP-conjugated antigen.ConclusionsZNPs possess good adjuvant potential as a vaccine carrier when combined with an antigen having a high affinity to ZNP. When complexed with ZBP-ScaA antigen, ZNPs could induce strong antibody responses as well as protective immunity against lethal challenges of O. tsutsugamushi. Therefore, application of ZNPs combined with a specific soluble antigen could be a promising strategy as a novel vaccine carrier system.

【 授权许可】

CC BY   
© The Author(s) 2016