Inhibition of H1N1 influenza virus infection by zinc oxide nanoparticles: another emerging application of nanomedicine

Journal of Biomedical Science

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[1] 0000 0001 0166 0922, grid.411705.6, Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;0000 0004 0418 0096, grid.411747.0, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran;0000 0004 4911 7066, grid.411746.1, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran;0000 0004 4911 7066, grid.411746.1, Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran;0000 0004 4911 7066, grid.411746.1, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran;0000 0004 4911 7066, grid.411746.1, Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran;0000 0004 4911 7066, grid.411746.1, Department of Medical Virology, Iran University of Medical Sciences, Tehran, Iran;

关键词: Antiviral activity;    Zinc oxide nanoparticle;    H1N1 influenza;    Polyethylene glycol;   

DOI  :  10.1186/s12929-019-0563-4

来源: publisher

【 摘 要 】

BackgroundCurrently available anti-influenza drugs are often associated with limitations such as toxicity and the appearance of drug-resistant strains. Therefore, there is a pressing need for the development of novel, safe and more efficient antiviral agents. In this study, we evaluated the antiviral activity of zinc oxide nanoparticles (ZnO-NPs) and PEGylated zinc oxide nanoparticles against H1N1 influenza virus.MethodsThe nanoparticles were characterized using the inductively coupled plasma mass spectrometry, x-ray diffraction analysis, and electron microscopy. MTT assay was applied to assess the cytotoxicity of the nanoparticles, and anti-influenza activity was determined by TCID50 and quantitative Real-Time PCR assays. To study the inhibitory impact of nanoparticles on the expression of viral antigens, an indirect immunofluorescence assay was also performed.ResultsPost-exposure of influenza virus with PEGylated ZnO-NPs and bare ZnO-NPs at the highest non-toxic concentrations could be led to 2.8 and 1.2 log10 TCID50 reduction in virus titer when compared to the virus control, respectively (P < 0.0001). At the highest non-toxic concentrations, the PEGylated and unPEGylated ZnO-NPs led to inhibition rates of 94.6 and 52.2%, respectively, which were calculated based on the viral loads. There was a substantial decrease in fluorescence emission intensity in viral-infected cell treated with PEGylated ZnO-NPs compared to the positive control.ConclusionsTaken together, our study indicated that PEGylated ZnO-NPs could be a novel, effective, and promising antiviral agent against H1N1 influenza virus infection, and future studies can be designed to explore the exact antiviral mechanism of these nanoparticles.

【 授权许可】

CC BY