Differential alterations of the concentrations of endocannabinoids and related lipids in the subcutaneous adipose tissue of obese diabetic patients

Lipids in Health and Disease

Research

Lucrezia Di Marino¹  Lidia Patti¹  Giuseppina Costabile¹  Lutgarda Bozzetto¹  Gabriele Riccardi¹  Giovanni Annuzzi¹  Angela A Rivellese¹  Vincenzo Di Marzo²  Roberta Verde²  Fabiana Piscitelli²  Stefania Petrosino²  Rosalba Giacco³ 

[1] Department of Clinical and Experimental Medicine, Federico II University, Naples, Italy;Endocannabinoid Research Group, Institute of Biomolecular Chemistry, CNR, Pozzuoli Naples, Italy;National Research Council, Avellino, Italy;

关键词: White Adipose Tissue;    Visceral Adipose Tissue;    Subcutaneous Adipose Tissue;    Anandamide;    Fatty Acid Amide Hydrolase;   

DOI  :  10.1186/1476-511X-9-43

 received in 2010-03-25, accepted in 2010-04-28,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe endocannabinoids, anandamide and 2-AG, are produced by adipocytes, where they stimulate lipogenesis via cannabinoid CB1 receptors and are under the negative control of leptin and insulin. Endocannabinoid levels are elevated in the blood of obese individuals and nonobese type 2 diabetes patients. To date, no study has evaluated endocannabinoid levels in subcutaneous adipose tissue (SAT) of subjects with both obesity and type 2 diabetes (OBT2D), characterised by similar adiposity and whole body insulin resistance and lower plasma leptin levels as compared to non-diabetic obese subjects (OB).Design and MethodsThe levels of anandamide and 2-AG, and of the anandamide-related PPARα ligands, oleoylethanolamide (OEA) and palmitoylethanolamide (PEA), in the SAT obtained by abdominal needle biopsy in 10 OBT2D, 11 OB, and 8 non-diabetic normal-weight (NW) subjects, were measured by liquid chromatography-mass spectrometry. All subjects underwent a hyperinsulinaemic euglycaemic clamp.ResultsAs compared to NW, anandamide, OEA and PEA levels in the SAT were 2-4.4-fold elevated (p < 0.05), and 2-AG levels 2.3-fold reduced (p < .05), in OBT2D but not in OB subjects. Anandamide, OEA and PEA correlated positively (p < .05) with SAT leptin mRNA and free fatty acid during hyperinsulinaemic clamp, and negatively with SAT LPL activity and plasma HDL-cholesterol, which were all specifically altered in OBT2D subjects.ConclusionsThe observed alterations emphasize, for the first time in humans, the potential different role and regulation of adipose tissue anandamide (and its congeners) and 2-AG in obesity and type 2 diabetes.

【 授权许可】

Unknown   
© Annuzzi et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.