A highly infectious Plasmodium yoelii parasite, bearing Plasmodium falciparum circumsporozoite protein

Malaria Journal

Research

Masao Yuda¹  Izumi Kaneko¹  Shiroh Iwanaga¹  Robert Mitchell²  Elizabeth H. Nardin²  Moriya Tsuji³  Tiffany Tsao³  Min Zhang⁴ 

[1] Department of Medical Zoology, Mie University Graduate School of Medicine, Tsu, Mie, Japan;Division of Parasitology, Department of Microbiology, New York University School of Medicine, New York, NY, USA;HIV and Malaria Vaccine Program, Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New York, NY, USA;HIV and Malaria Vaccine Program, Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New York, NY, USA;Department of Pathology, New York University School of Medicine, New York, NY, USA;

关键词: PfCSP/Py;    Circumsporozoite protein;    Plasmodium falciparum;    Plasmodium yoelii;   

DOI  :  10.1186/s12936-016-1248-z

 received in 2016-01-28, accepted in 2016-03-30,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundPlasmodium circumsporozoite protein (CSP) is a major surface antigen present in the sporozoite (Spz) stage of a malaria parasite. RTS, S vaccine, the most clinically advanced malaria vaccine, consists of a large portion of Plasmodium falciparum CSP (PfCSP). A highly infectious, recombinant rodent malaria, Plasmodium yoelii parasite bearing a full-length PfCSP, PfCSP/Py Spz, was needed as a tool to evaluate the role of PfCSP in mediating, protective, anti-malaria immunity in a mouse model.MethodsA transgenic parasite, PfCSP/Py Spz, was generated by inserting a construct expressing the PfCSP at the locus of the P. yoelii CSP gene by double cross-over homologous recombination. Then the biological and protective properties of PfCSP/Py Spz were determined.ResultsThis PfCSP/Py parasite produced up to 30,000 Spz in mosquito salivary glands, which is equal or even higher than the number of Spz produced by wild-type P. yoelii parasites. Five bites of PfCSP/Py-infected mosquitoes could induce blood infection in BALB/c mice.ConclusionsThe current study has demonstrated a successful establishment of a transgenic P. yoelii parasite clone that is able to express a full-length PfCSP, PfCSP/Py parasite. Importantly, this PfCSP/Py parasite can be as infectious as the wild-type P. yoelii parasite both in mosquito vector and in mouse, a mammalian host. A new transgenic parasite that expresses a full-length PfCSP may become a useful tool for researchers to investigate immunity against PfCSP in a mouse model.

【 授权许可】

CC BY   
© Zhang et al. 2016