Early alterations in vascular contractility associated to changes in fatty acid composition and oxidative stress markers in perivascular adipose tissue

Cardiovascular Diabetology

Original Investigation

Oscar R Rebolledo¹  Juan J Gagliardino¹  María E García¹  Alejandro Rebolledo²  Laura Rimorini²  Ana R Roldán Palomo²  Carlos A Marra³ 

[1] CENEXA - Centro de Endocrinología Experimental y Aplicada, UNLP-CONICET LA PLATA, Centro Colaborador OPS/OMS), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina;GINFIV - Grupo de Investigación en Fisiología Vascular, Grupo Vinculado a CENEXA (UNLP - CONICET LA PLATA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina;INIBIOLP - Instituto de Investigaciones Bioquímicas de La Plata, UNLP-CONICET LA PLATA, Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina;

关键词: Nitric Oxide;    Aortic Ring;    Commercial Diet;    Fructosamine;    Abdominal Adipose Tissue;   

DOI  :  10.1186/1475-2840-9-65

 received in 2010-08-02, accepted in 2010-10-21,  发布年份 2010

来源: Springer

【 摘 要 】

AimTo test the early effect of fructose-induced changes in fatty acid composition and oxidative stress markers in perivascular adipose tissue (PVAT) upon vascular contractility.MethodsAdult male Wistar rats were fed a commercial diet without (CD) or with 10% fructose (FRD) in the drinking water for 3 weeks. We measured plasma metabolic parameters, lipid composition and oxidative stress markers in aortic PVAT. Vascular contractility was measured in aortic rings sequentially, stimulated with serotonin (5-HT) and high K+-induced depolarization using intact and thereafter PVAT-deprived rings.ResultsComparable body weights were recorded in both groups. FRD rats had increased plasma triglyceride and fructosamine levels. Their PVAT had an increased saturated to mono- or poly-unsaturated fatty acid ratio, a significant decrease in total superoxide dismutase and glutathione peroxidase activities and in the total content of glutathione. Conversely, lipid peroxidation (TBARS), nitric oxide content, and gluthathione reductase activity were significantly higher, indicating an increase in oxidative stress. In aortic rings, removal of PVAT increased serotonin-induced contractions, but the effect was significantly lower in rings from FRD rats. This effect was no longer observed when the two contractions were performed in PVAT-deprived rings. PVAT did not affect the contractions triggered by high K+-induced depolarization either in CD or FRD rats.ConclusionsFRD induces multiple metabolic and endocrine systemic alterations which also alter PVAT and the vascular relaxant properties of this tissue. The changes in PVAT would affect its paracrine modulation of vascular function.

【 授权许可】

CC BY   
© Rebolledo et al; licensee BioMed Central Ltd. 2010