Vasorelaxant effect of Prunus yedoensis bark

BMC Complementary and Alternative Medicine

Research Article

Ho-Young Choi¹  Youngmin Bu¹  Gabsik Yang¹  Kyungjin Lee¹  Hocheol Kim¹  Inhye Ham¹  Mihwa Lee¹ 

[1] Department of Herbology, College of Korean Medicine, Kyung Hee University, 1 Hoegi-Dong, 130–701, Dongdaemun-Gu, Seoul, Republic of Korea;

关键词: Nitric Oxide;    Methylene Blue;    Glibenclamide;    Relaxant Effect;    Aortic Ring;   

DOI  :  10.1186/1472-6882-13-31

 received in 2012-08-31, accepted in 2013-01-31,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundPrunus yedoensis Matsum. is used as traditional medicine—‘Yaeng-Pi’ or ‘Hua-Pi’—in Japan and Korea. However, no studies have examined the pharmacological activities of the P. yedoensis bark. Only the antioxidant and antiviral activities of P. yedoensis fruit and the anti-hyperglycaemic effect of P. yedoensis leaf have been investigated. While studying the antihypertensive effects of several medicinal plants, we found that a methanol extract of P. yedoensis bark (MEPY) had distinct vasorelaxant effects on rat aortic rings.MethodsThe aortic rings were removed from Sprague–Dawley rats and suspended in organ chambers containing 10 ml Krebs-Henseleit solution. The aortic rings were placed between 2 tungsten stirrups and connected to an isometric force transducer. Changes in tension were recorded via isometric transducers connected to a data acquisition system.ResultsMEPY relaxed the contraction induced by phenylephrine (PE) both in endothelium-intact and endothelium-denuded aortic rings concentration dependently. However, the vasorelaxant effects of MEPY on endothelium-denuded aortic rings were lower than endothelium-intact aortic rings. The vasorelaxant effects of MEPY on endothelium-intact aortic rings were reduced by pre-treatment with l-NAME, methylene blue, or ODQ. However, pre-treatment with indomethacin, atropine, glibenclamide, tetraethylammonium, or 4-aminopyridine had no affection. In addition, MEPY inhibited the contraction induced by extracellular Ca2+ in endothelium-denuded rat thoracic aorta rings pre-contracted by PE (1 μM) or KCl (60 mM) in Ca2+-free solution.ConclusionsOur results suggest that MEPY exerts its vasorelaxant effects via the activation of NO formation by means of l-Arg and NO-cGMP pathways and via the blockage of extracellular Ca2+ channels.

【 授权许可】

Unknown   
© Lee et al; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.