Carbohydrate functionalization of silver nanoparticles modulates cytotoxicity and cellular uptake

Journal of Nanobiotechnology

Research

Chian-Hui Lai¹  Guillermo Orts-Gil¹  Peter H Seeberger²  David C Kennedy³  Andrea Haase⁴  Andreas Luch⁴  Larissa Müller⁵ 

[1] Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces (MPIKG), 14476, Potsdam, Germany;Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces (MPIKG), 14476, Potsdam, Germany;Institute for Chemistry and Biochemistry, Free University Berlin, Arnimallee 22, 14195, Berlin, Germany;Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces (MPIKG), 14476, Potsdam, Germany;National Research Council Canada (CNRC), 100 Sussex Drive, Ottawa, Ontario, Canada;Departments Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), 10589, Berlin, Germany;Division 1.1 Inorganic Trace Analysis, Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Straße 11, 12489, Berlin, Germany;

关键词: Silver;    Nanoparticles;    Carbohydrates;    Nanotoxicology;    Bio-interfaces;   

DOI  :  10.1186/s12951-014-0059-z

 received in 2014-08-06, accepted in 2014-12-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundIncreasing use of silver nanoparticles (Ag-NPs) in various products is resulting in a greater likelihood of human exposure to these materials. Nevertheless, little is still known about the influence of carbohydrates on the toxicity and cellular uptake of nanoparticles.MethodsAg-NPs functionalized with three different monosaccharides and ethylene glycol were synthesized and characterised. Oxidative stress and toxicity was evaluated by protein carbonylation and MTT assay, respectively. Cellular uptake was evaluated by confocal microscopy and ICP-MS.ResultsAg-NPs coated with galactose and mannose were considerably less toxic to neuronal-like cells and hepatocytes compared to particles functionalized by glucose, ethylene glycol or citrate. Toxicity correlated to oxidative stress but not to cellular uptake.ConclusionsCarbohydrate coating on silver nanoparticles modulates both oxidative stress and cellular uptake, but mainly the first has an impact on toxicity. These findings provide new perspectives on modulating the bioactivity of Ag-NPs by using carbohydrates.

【 授权许可】

Unknown   
© Kennedy et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.