Carbohydrate functionalization of silver nanoparticles modulates cytotoxicity and cellular uptake

Journal of Nanobiotechnology

Peter H Seeberger¹  Andreas Luch⁵  Andrea Haase⁵  Larissa Müller²  Chian-Hui Lai³  Guillermo Orts-Gil³  David C Kennedy⁴ 

[1] Institute for Chemistry and Biochemistry, Free University Berlin, Arnimallee 22, Berlin, 14195, Germany;Division 1.1 Inorganic Trace Analysis, Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Straße 11, Berlin, 12489, Germany;Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces (MPIKG), Potsdam, 14476, Germany;National Research Council Canada (CNRC), 100 Sussex Drive, Ottawa, Ontario, Canada;Departments Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), Berlin, 10589, Germany

关键词: Bio-interfaces;    Nanotoxicology;    Carbohydrates;    Nanoparticles;    Silver;   

Others  :  1139284
DOI  :  10.1186/s12951-014-0059-z

 received in 2014-08-06, accepted in 2014-12-11,  发布年份 2014

【 摘 要 】

Background

Increasing use of silver nanoparticles (Ag-NPs) in various products is resulting in a greater likelihood of human exposure to these materials. Nevertheless, little is still known about the influence of carbohydrates on the toxicity and cellular uptake of nanoparticles.

Methods

Ag-NPs functionalized with three different monosaccharides and ethylene glycol were synthesized and characterised. Oxidative stress and toxicity was evaluated by protein carbonylation and MTT assay, respectively. Cellular uptake was evaluated by confocal microscopy and ICP-MS.

Results

Ag-NPs coated with galactose and mannose were considerably less toxic to neuronal-like cells and hepatocytes compared to particles functionalized by glucose, ethylene glycol or citrate. Toxicity correlated to oxidative stress but not to cellular uptake.

Conclusions

Carbohydrate coating on silver nanoparticles modulates both oxidative stress and cellular uptake, but mainly the first has an impact on toxicity. These findings provide new perspectives on modulating the bioactivity of Ag-NPs by using carbohydrates.

【 授权许可】

   
2014 Kennedy et al.; licensee BioMed Central.

【 图 表 】

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