Forkhead-box transcription factor 1 affects the apoptosis of natural regulatory T cells by controlling Aven expression

BMC Immunology

Research Article

Hong Liu¹  Xiongfei Wu¹  Zhitao Cai¹ 

[1] Department of Nephrology, Southwest Hospital, Third Military Medical University, 400038, Chongqing, People’s Republic of China;

关键词: Treg cells;    Foxo1;    Aven;    Apoptosis;   

DOI  :  10.1186/s12865-017-0198-8

 received in 2016-10-04, accepted in 2017-02-16,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundRegulatory T (Treg) cells play important roles in autoimmune diseases, cancer, and organ transplantation. Forkhead box protein o1 (Foxo1) and IL-7Rα(CD127) are closely related to the homeostasis of Treg cells. However, the mechanism underlying Treg proliferation and activation remains unclear. Here, we evaluated how the over-expression of Foxo1 affects Treg cell proliferation via intracellular signaling. nTreg cells were transfected separately with Foxo1 and Aven small-interfering RNA (siRNA) or over-expression plasmid. The expression of signaling pathway genes and CD127 was confirmed using RT-qPCR and western blot analysis. The expression of cell surface molecules and apoptosis was confirmed by Flow Cytometry 3-(4, 5-Dimethylthiazol-2-yl) 2,5- diphenyltetrazolium bromide for cell proliferation assays.ResultsFoxo1 strengthened the proliferative ability of Treg cells by activating IL-7/CD127 signaling. In addition, Foxo1 suppressed Treg cell apoptosis by regulating Aven expression.ConclusionsThe results in this study indicated that Foxo1 is a positive regulatory factor for the proliferation and activity of Treg cells. Foxo1 might be a potential target for the activation of nTreg cells in vivo and in vitro.

【 授权许可】

CC BY   
© The Author(s). 2017