Antibody levels against GLURP R2, MSP1 block 2 hybrid and AS202.11 and the risk of malaria in children living in hyperendemic (Burkina Faso) and hypo-endemic (Ghana) areas

Malaria Journal

Research

Sodiomon B. Sirima¹  Issa Nebie¹  Amidou Diarra¹  Michael Theisen²  Fareed K. N. Arthur³  Giampietro Corradin⁴  David R. Cavanagh⁵  Emmanuel K. Dickson⁶  Bright Adu⁶  Daniel Dodoo⁶  Mariama K. Cherif⁷  Samuel Bosomprah⁸ 

[1] Centre National de Recherche et de Formation sur le paludisme, Ouagadougou, Burkina Faso;Department for Congenital Disorders, Statens Serum Institut, Copenhagen, Denmark;Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;Department of Biochemistry, University of Lausanne, Epalinges, Switzerland;Institute of Cell, Animal and Population Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland, UK;Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana;Polytechnic University of BoboDioulasso, Bobo-Dioulasso, Burkina Faso;Centre National de Recherche et de Formation sur le paludisme, Ouagadougou, Burkina Faso;School of Public Health, University of Ghana, Legon, Ghana;

关键词: Malaria;    Antibodies;    GLURP R2;    MSP1 block 2 hybrid;    AS202.11;    Hyperendemic;    Hypo-endemic;    Transmission intensity;   

DOI  :  10.1186/s12936-016-1146-4

 received in 2015-11-28, accepted in 2016-02-04,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundDifferences in parasite transmission intensity influence the process of acquisition of host immunity to Plasmodium falciparum malaria and ultimately, the rate of malaria related morbidity and mortality. Potential vaccines being designed to complement current intervention efforts therefore need to be evaluated against different malaria endemicity backgrounds.MethodsThe associations between antibody responses to the chimeric merozoite surface protein 1 block 2 hybrid (MSP1 hybrid), glutamate-rich protein region 2 (GLURP R2) and the peptide AS202.11, and the risk of malaria were assessed in children living in malaria hyperendemic (Burkina Faso, n = 354) and hypo-endemic (Ghana, n = 209) areas. Using the same reagent lots and standardized protocols for both study sites, immunoglobulin (Ig) M, IgG and IgG sub-class levels to each antigen were measured by ELISA in plasma from the children (aged 6–72 months). Associations between antibody levels and risk of malaria were assessed using Cox regression models adjusting for covariates.ResultsThere was a significant association between GLURP R2 IgG3 and reduced risk of malaria after adjusting age of children in both the Burkinabe (hazard ratio 0.82; 95 % CI 0.74–0.91, p < 0.0001) and the Ghanaian (HR 0.48; 95 % CI 0.25–0.91, p = 0.02) cohorts. MSP1 hybrid IgM was associated (HR 0.85; 95 % CI 0.73–0.98, p = 0.02) with reduced risk of malaria in Burkina Faso cohort while IgG against AS202.11 in the Ghanaian children was associated with increased risk of malaria (HR 1.29; 95 % CI 1.01–1.65, p = 0.04).ConclusionThese findings support further development of GLURP R2 and MSP1 block 2 hybrid, perhaps as a fusion vaccine antigen targeting malaria blood stage that can be deployed in areas of varying transmission intensity.

【 授权许可】

CC BY   
© Adu et al. 2016