PPM1D silencing by RNA interference inhibits the proliferation of lung cancer cells

World Journal of Surgical Oncology

Research

Mingsong Wang¹  Yongxin Li²  Sekwon Kim²  E Song³  Chen Zhang⁴  Xianzhen Chen⁴  Yuanzhuo Chen⁴  Hu Peng⁴  Xiaoqing Liu⁴ 

[1] Department of Cardiothoracic Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, 200092, Shanghai, China;Department of Marine Bio Convergence Science, Specialized Graduate School Science and Technology Convergence, Pukyong National University, 608-737, Busan, Republic of Korea;Lixiang Eye Hospital of Soochow University, 215000, Suzhou, Jiangsu, China;Shanghai Tenth People’s Hospital, Tongji University School of Medicine, 200072, Shanghai, China;

关键词: PPM1D;    lung cancer;    shRNA;    cell proliferation;    cell cycle;   

DOI  :  10.1186/1477-7819-12-258

 received in 2014-05-17, accepted in 2014-07-20,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundPPM1D (protein phosphatase, Mg2+/Mn2+ dependent, 1D) has been reported to be involved in multiple human tumors. This study was designed to investigate the functional role of PPM1D in lung cancer cells.MethodsExpression levels of PPM1D were analyzed in A549 and H1299 cells by real-time PCR and Western blotting. Lentivirus-mediated short hairpin RNA (shRNA) was used to knock down PPM1D expression in both cell lines. The effects of PPM1D on lung cancer cell growth were investigated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), colony formation and flow cytometry assays.ResultsKnockdown of PPM1D in lung cancer cells resulted in decreased cell proliferation and impaired colony formation ability. Moreover, flow cytometry analysis showed that knockdown of PPM1D arrested cell cycle at the G0/G1 phase. Furthermore, PPM1D silencing downregulated the expression of cyclin B1 in H1299 cells. Therefore, it is reasonable to speculate that the mechanisms by which PPM1D knockdown alleviates cell growth may be partly via the induction of cell cycle arrest due to the suppression of cyclin B1.ConclusionsThese results suggest that PPM1D silencing by RNA interference (RNAi) may be a potential therapeutic approach for the treatment of lung cancer.

【 授权许可】

Unknown   
© Zhang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.