PPM1D silencing by RNA interference inhibits the proliferation of lung cancer cells

World Journal of Surgical Oncology

Hu Peng⁴  Sekwon Kim¹  Xiaoqing Liu⁴  E Song²  Yongxin Li¹  Xianzhen Chen⁴  Mingsong Wang³  Yuanzhuo Chen⁴  Chen Zhang⁴ 

[1] Department of Marine Bio Convergence Science, Specialized Graduate School Science and Technology Convergence, Pukyong National University, Busan 608-737, Republic of Korea;Lixiang Eye Hospital of Soochow University, Suzhou, Jiangsu 215000, China;Department of Cardiothoracic Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China;Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China

关键词: cell cycle;    cell proliferation;    shRNA;    lung cancer;    PPM1D;   

Others  :  1148300
DOI  :  10.1186/1477-7819-12-258

 received in 2014-05-17, accepted in 2014-07-20,  发布年份 2014

【 摘 要 】

Background

PPM1D (protein phosphatase, Mg²⁺/Mn²⁺ dependent, 1D) has been reported to be involved in multiple human tumors. This study was designed to investigate the functional role of PPM1D in lung cancer cells.

Methods

Expression levels of PPM1D were analyzed in A549 and H1299 cells by real-time PCR and Western blotting. Lentivirus-mediated short hairpin RNA (shRNA) was used to knock down PPM1D expression in both cell lines. The effects of PPM1D on lung cancer cell growth were investigated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), colony formation and flow cytometry assays.

Results

Knockdown of PPM1D in lung cancer cells resulted in decreased cell proliferation and impaired colony formation ability. Moreover, flow cytometry analysis showed that knockdown of PPM1D arrested cell cycle at the G₀/G₁ phase. Furthermore, PPM1D silencing downregulated the expression of cyclin B1 in H1299 cells. Therefore, it is reasonable to speculate that the mechanisms by which PPM1D knockdown alleviates cell growth may be partly via the induction of cell cycle arrest due to the suppression of cyclin B1.

Conclusions

These results suggest that PPM1D silencing by RNA interference (RNAi) may be a potential therapeutic approach for the treatment of lung cancer.

【 授权许可】

   
2014 Zhang et al.; licensee BioMed Central Ltd.

【 图 表 】

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