Evaluating the feasibility of using insecticide quantification kits (IQK) for estimating cyanopyrethroid levels for indoor residual spraying in Vanuatu

Malaria Journal

Research

Teunis Eggelte¹  Folasade Oladepo²  Mark JI Paine²  Janet Hemingway²  Hanafy Ismail²  John C Morgan²  Harparkash Kaur³  James Amon⁴  Harry Iata⁴  Tanya L Russell⁵ 

[1] Institute for Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands;Liverpool School of Tropical Medicine, Pembroke Place, L3 5QA, Liverpool, UK;London School of Hygiene and Tropical Medicine, London, UK;National Vector Borne Disease Control Programme, Ministry of Health, Tafea Province, Vanuatu;University of Queensland, School of Population Health, Australian Centre for Tropical and International Health, Pacific Malaria Initiative Support Centre, 4006, Herston, Australia;James Cook University, Faculty of Medicine, Health and Molecular Sciences, Queensland Tropical Health Alliance, 4870, Cairns, Australia;

关键词: Colorimetric assay;    Insecticide residual spray (IRS);    Vanuatu;    Insecticide quantification kit;   

DOI  :  10.1186/1475-2875-13-178

 received in 2014-02-06, accepted in 2014-04-27,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe quality of routine indoor residual spraying (IRS) operations is rarely assessed because of the limited choice of methods available for quantifying insecticide content in the field. This study, therefore, evaluated a user-friendly, rapid colorimetric assay for detecting insecticide content after routine IRS operations were conducted.MethodsThis study was conducted in Tafea Province, Vanuatu. Routine IRS was conducted with lambda cyhalothrin. Two methods were used to quantify the IRS activities: 1) pre-spray application of small felt pads and 2) post-spray removal of insecticide with adhesive. The insecticide content was quantified using a colorimetric assay (Insecticide Quantification Kit [IQK]), which involved exposing each sample to the test reagents for 15 mins. The concentration of insecticide was indicated by the depth of red colour.ResultsThe IQK proved simple to perform in the field and results could be immediately interpreted by the programme staff. The insecticide content was successfully sampled by attaching felt pads to the house walls prior to spraying. The IRS operation was well conducted, with 83% of houses being sprayed at the target dose (20 – 30 mg AI/m2). The average reading across all houses was 24.4 ± 1.5 mg AI/m2. The results from the felt pads applied pre-spray were used as a base to compare methods for sampling insecticide from walls post-spray. The adhesive of Sellotape did not collect adequate samples. However, the adhesive of the felt pads provided accurate samples of the insecticide content on walls.ConclusionThe IQK colorimetric assay proved to be a useful tool that was simple to use under realistic field conditions. The assay provided rapid information on IRS spray dynamics and spray team performance, facilitating timely decision making and reporting for programme managers. The IQK colorimetric assay will have direct applications for routine quality control in malaria control programmes globally and has the potential to improve the efficacy of vector control operations.

【 授权许可】

Unknown   
© Russell et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.