Identification of genomic regions involved in tolerance to drought stress and drought stress induced leaf senescence in juvenile barley

BMC Plant Biology

Research Article

Frank F Ordon¹  Klaus K Humbeck²  Matthias M Enders³  Christiane C Balko⁴  Gwendolin G Wehner⁴ 

[1] Interdisciplinary Center for Crop Plant Research (IZN), Hoher Weg 8, 06120, Halle (Saale), Germany;Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany;Interdisciplinary Center for Crop Plant Research (IZN), Hoher Weg 8, 06120, Halle (Saale), Germany;Martin-Luther-University Halle-Wittenberg, Institute of Biology, Weinbergweg 10, 06120, Halle (Saale), Germany;Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany;Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Rudolf-Schick-Platz 3, 18190, Sanitz, Germany;Interdisciplinary Center for Crop Plant Research (IZN), Hoher Weg 8, 06120, Halle (Saale), Germany;

关键词: Barley;    Leaf senescence;    Drought stress;    GWAS;    QTL;   

DOI  :  10.1186/s12870-015-0524-3

 received in 2015-02-16, accepted in 2015-05-11,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundPremature leaf senescence induced by external stress conditions, e.g. drought stress, is a main factor for yield losses in barley. Research in drought stress tolerance has become more important as due to climate change the number of drought periods will increase and tolerance to drought stress has become a goal of high interest in barley breeding. Therefore, the aim is to identify quantitative trait loci (QTL) involved in drought stress induced leaf senescence and drought stress tolerance in early developmental stages of barley (Hordeum vulgare L.) by applying genome wide association studies (GWAS) on a set of 156 winter barley genotypes.ResultsAfter a four weeks stress period (BBCH 33) leaf colour as an indicator of leaf senescence, electron transport rate at photosystem II, content of free proline, content of soluble sugars, osmolality and the aboveground biomass indicative for drought stress response were determined in the control and stress variant in greenhouse pot experiments. Significant phenotypic variation was observed for all traits analysed. Heritabilities ranged between 0.27 for osmolality and 0.61 for leaf colour in stress treatment and significant effects of genotype, treatment and genotype x treatment were estimated for most traits analysed. Based on these phenotypic data and 3,212 polymorphic single nucleotide polymorphisms (SNP) with a minor allele frequency >5 % derived from the Illumina 9 k iSelect SNP Chip, 181 QTL were detected for all traits analysed. Major QTLs for drought stress and leaf senescence were located on chromosome 5H and 2H. BlastX search for associated marker sequences revealed that respective SNPs are in some cases located in proteins related to drought stress or leaf senescence, e.g. nucleotide pyrophosphatase (AVP1) or serine/ threonin protein kinase (SAPK9).ConclusionsGWAS resulted in the identification of many QTLs involved in drought stress and leaf senescence of which two major QTLs for drought stress and leaf senescence were located on chromosome 5H and 2H. Results may be the basis to incorporate breeding for tolerance to drought stress or leaf senescence in barley breeding via marker based selection procedures.

【 授权许可】

Unknown   
© Wehner et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.