Use of tuf as a target for sequence-based identification of Gram-positive cocci of the genus Enterococcus, Streptococcus, coagulase-negative Staphylococcus, and Lactococcus

Annals of Clinical Microbiology and Antimicrobials

Research

Baoyu Li¹  Juan Xing¹  Xuerui Li¹  Jixing Liu¹  Pu Wang² 

[1] State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of the Ministry of Agriculture; Key Laboratory of Veterinary Public Health of the Ministry of Agriculture; Animal Infectious Diseases Research Laboratory, Lanzhou Veterinary Research Institute, CAAS, 730046, Lanzhou, China;The Wistar Institute, 19104, Philadelphia, PA, USA;

关键词: Enterococcus;    Streptococcus;    Staphylococcus;    Lactococcus;    tuf;    species identification;   

DOI  :  10.1186/1476-0711-11-31

 received in 2012-08-24, accepted in 2012-11-15,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundAccurate identification of isolates belonging to genus Enterococcus, Streptococcus, coagulase-negative Staphylococcus, and Lactococcus at the species level is necessary to provide a better understanding of their pathogenic potential, to aid in making clinical decisions, and to conduct epidemiologic investigations,especially when large blind samples must be analyzed. It is useful to simultaneously identify species in different genera using a single primer pair.MethodsWe developed a primer pair based on the tuf gene (encoding elongation factor) sequence to identify 56 Gram-positive cocci isolates.ResultsThe target sequences were amplified from all 56 samples. The sequencing results and the phylogenetic tree derived from the partial tuf gene sequences identified the isolates as three enterococcal species, two lactococcal species, two staphylococcal species, and six streptococcal species, as well as eight isolates that were novel species of the genus Streptococcus. Partial gene sequence analysis of the sodA, dnaK, and 16S RNA genes confirmed the results obtained by tuf gene sequencing.ConclusionBased on the uniform amplification of the tuf gene from all samples and the ability to identify all isolates at both the genus and species levels, we conclude that the primer pair developed in this research provides a powerful tool for identifying these organisms in clinical laboratories where large blind samples are used.

【 授权许可】

Unknown   
© Li et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.