Acidification changes affect the inflammasome in human nucleus pulposus cells

Journal of Inflammation

Research

Francesco Travascio¹  Frank J. Brand²  Harmanpreet Kaur²  Mahtab Forouzandeh²  Juan Pablo de Rivero Vaccari³ 

[1] Biomechanics Research Laboratory, Department of Industrial Engineering, University of Miami, 33146, Coral Gables, FL, USA;Department of Neurological Surgery, The Miami Project to Cure Paralysis, Miller School of Medicine, University of Miami, 33136, Miami, FL, USA;Department of Neurological Surgery, The Miami Project to Cure Paralysis, Miller School of Medicine, University of Miami, 33136, Miami, FL, USA;Department of Neurological Surgery, Lois Pope LIFE Center, 1095 NW 14th Terrace, 3-25JJ, 33136-1060, Miami, FL, USA;

关键词: Innate Immunity;    Intervertebral disc degeneration;    Caspase-1;    Inflammasome;    Inflammation;   

DOI  :  10.1186/s12950-016-0137-0

 received in 2016-04-28, accepted in 2016-08-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundInterleukin (IL)-1β is involved in the pathology of intervertebral disc degeneration. Under normal conditions, IL-1β is present in cells in an inactive form (pro-IL-1β). However, under pathological conditions, pro-IL-1β is turned into its active form (IL-1β) by the inflammasome, a multi-protein complex of the innate immune response that activates caspase-1. Under conditions of degeneration, the disc experiences an environment of increased acidification. However, the implications of acidification on the innate immune response remain poorly explored.MethodsHere we have studied how pH changes in human nucleus pulposus cells affect inflammasome activation by immunoblot analysis of protein lysates obtained from nucleus pulposus cells that were exposed to different pH levels in culture.ResultsIn this study, we have found that in nucleus pulposus cells, with increased acidification, there was a decrease in inflammasome activation consistent with lower levels of active IL-1β. However, this effect at a pH of 6.5, the lowest pH level tested, was abrogated when cells were treated with IL-1β.ConclusionsTaken together, these findings suggest that the inflammatory response through IL-1β experienced by the human disc is not initiated in nucleus pulposus cells when the stimulus is acidification.

【 授权许可】

CC BY   
© The Author(s). 2016