Next-generation sequencing: recent applications to the analysis of colorectal cancer

Journal of Translational Medicine

Review

Valentina Mastroiaco¹  Francesca Zazzeroni¹  Alessandra Tessitore¹  Edoardo Alesse¹  Chiara Compagnoni¹  Antinisca Di Marco¹  Daria Capece²  Carlo Capalbo³  Filippo Del Vecchio⁴ 

[1] Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100, L’Aquila, Italy;Department of Medicine, Centre for Cell Signaling and Inflammation, Imperial College London, W12 0NN, London, UK;Department of Molecular Medicine, La Sapienza University, 00161, Rome, Italy;Division of Cancer Sciences, University of Southampton, SO16 6YD, Southampton, Hampshire, UK;

关键词: Next-generation sequencing;    Colorectal cancer;    Precision medicine;    Targeted therapy;   

DOI  :  10.1186/s12967-017-1353-y

 received in 2017-07-31, accepted in 2017-11-27,  发布年份 2017

来源: Springer

【 摘 要 】

Since the establishment of the Sanger sequencing method, scientists around the world focused their efforts to progress in the field to produce the utmost technology. The introduction of next-generation sequencing (NGS) represents a revolutionary step and promises to lead to massive improvements in our understanding on the role of nucleic acids functions. Cancer research began to use this innovative and highly performing method, and interesting results started to appear in colorectal cancer (CRC) analysis. Several studies produced high-quality data in terms of mutation discovery, especially about actionable or less frequently mutated genes, epigenetics, transcriptomics. Analysis of results is unveiling relevant perspectives aiding to evaluate the response to therapies. Novel evidences have been presented also in other directions such as gut microbiota or CRC circulating tumor cells. However, despite its unquestioned potential, NGS poses some issues calling for additional studies. This review intends to offer a view of the state of the art of NGS applications to CRC through examination of the most important technologies and discussion of recent published results.

【 授权许可】

CC BY   
© The Author(s) 2017