Journal of Translational Medicine | |
Next-generation sequencing: recent applications to the analysis of colorectal cancer | |
Review | |
Valentina Mastroiaco1  Francesca Zazzeroni1  Alessandra Tessitore1  Edoardo Alesse1  Chiara Compagnoni1  Antinisca Di Marco1  Daria Capece2  Carlo Capalbo3  Filippo Del Vecchio4  | |
[1] Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100, L’Aquila, Italy;Department of Medicine, Centre for Cell Signaling and Inflammation, Imperial College London, W12 0NN, London, UK;Department of Molecular Medicine, La Sapienza University, 00161, Rome, Italy;Division of Cancer Sciences, University of Southampton, SO16 6YD, Southampton, Hampshire, UK; | |
关键词: Next-generation sequencing; Colorectal cancer; Precision medicine; Targeted therapy; | |
DOI : 10.1186/s12967-017-1353-y | |
received in 2017-07-31, accepted in 2017-11-27, 发布年份 2017 | |
来源: Springer | |
【 摘 要 】
Since the establishment of the Sanger sequencing method, scientists around the world focused their efforts to progress in the field to produce the utmost technology. The introduction of next-generation sequencing (NGS) represents a revolutionary step and promises to lead to massive improvements in our understanding on the role of nucleic acids functions. Cancer research began to use this innovative and highly performing method, and interesting results started to appear in colorectal cancer (CRC) analysis. Several studies produced high-quality data in terms of mutation discovery, especially about actionable or less frequently mutated genes, epigenetics, transcriptomics. Analysis of results is unveiling relevant perspectives aiding to evaluate the response to therapies. Novel evidences have been presented also in other directions such as gut microbiota or CRC circulating tumor cells. However, despite its unquestioned potential, NGS poses some issues calling for additional studies. This review intends to offer a view of the state of the art of NGS applications to CRC through examination of the most important technologies and discussion of recent published results.
【 授权许可】
CC BY
© The Author(s) 2017
【 预 览 】
Files | Size | Format | View |
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RO202311100989179ZK.pdf | 943KB | download |
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