DJ-1 is activated in medulloblastoma and is associated with cell proliferation and differentiation

World Journal of Surgical Oncology

Research

Jia-ping Lin¹  Zhi Li²  Bin Li²  Yang Li²  Bin-cai Pan³  Xiao-ying Tian⁴ 

[1] Department of Neurosurgery, the First Affiliated Hospital, Sun Yat-sen University, 58, Zhongshan Road II, 510080, Guangzhou, China;Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 58, Zhongshan Road II, 510080, Guangzhou, China;Department of Pathology, Tongjiang Hospital of Guangdong, Nanguo Road East, 528300, Shunde District, Foshan, China;School of Chinese Medicine, Hong Kong Baptist University, 7, Baptist University Road, Kowloon Tong, Hong Kong, China;

关键词: Medulloblastoma;    DJ-1;    PI3K/Akt pathway;    PTEN;    Prognosis;   

DOI  :  10.1186/1477-7819-12-373

 received in 2014-06-04, accepted in 2014-11-18,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundDJ-1 is a key regulator in human tumorigenesis, including brain malignancies. The mechanisms by which DJ-1 contributes to the pathogenesis of medulloblastoma (MB) remain unclear, and its impact on the prognosis for patients with MB has not been identified. The aim of this study was to determine whether the DJ-1 protein is associated with tumorigenesis of MBs, and whether DJ-1 is a valuable factor for predicting the prognosis of patients with MB.MethodsWe collected 66 pairs of MB and adjacent normal cerebellum samples. Expression of DJ-1, Ser 473-phosphorylated-Akt (p-Akt), PTEN, and Ki-67 (MIB-1) was detected by immunohistochemical staining, and the correlation of these immunostaining results with the clinicopathological features of patients with MB was determined.ResultsHigh DJ-1 expression (48.5%, 32/66) in tumor cells of MBs was significantly associated with the classic MB variant (P = 0.003), high proliferative activity (P = 0.002) and undifferentiated tumor (P = 0.001), whereas high p-Akt expression (56.1%, 37/66) was associated with tumor metastasis stage (P = 0.007), undifferentiated tumor (P = 0.007), and high-risk tumor (P = 0.002). High DJ-1 expression also correlated with high p-Akt expression and high MIB-1 index. However, only high levels of DJ-1(P = 0.009) and high MIB-1 index (P = 0.001) were strong independent prognostic factors associated with worse overall survival.ConclusionsAlthough the validity of the preliminary data in this study needs to be confirmed by a larger number of cases, our study indicates that DJ-1, PTEN, and p-Akt might play important roles in cell proliferation and differentiation of MBs. The evaluation of expression of DJ-1 and related proteins might be useful for predicting the prognosis of patients with MB.

【 授权许可】

CC BY   
© Lin et al.; licensee BioMed Central Ltd. 2014