Propofol inhibits the release of interleukin-6, 8 and tumor necrosis factor-α correlating with high-mobility group box 1 expression in lipopolysaccharides-stimulated RAW 264.7 cells

BMC Anesthesiology

Research Article

Yijuan Sun¹  Zurong Hu¹  Jie Jia²  Xiangcai Ruan³  Yi Li⁴ 

[1] Department of Anesthesiology, Guangdong Women and Children Hospital, Guangzhou, China;Department of Anesthesiology, The First Affiliated Hospital of Jinan University, Guangzhou, China;Department of Anesthesiology, Guangdong Women and Children Hospital, Guangzhou, China;Department of Anesthesiology, The First Affiliated Hospital of Jinan University, Guangzhou, China;Department of Anesthesiology, Guangzhou First People’s Hospital, Affiliated Hospital of Guangzhou Medical University, No. 1 Panfu Road, 510180, Guangzhou, China;Department of Gynecology, Guangdong Women and Children Hospital, Guangzhou, China;

关键词: Lipopolysaccharide;    Propofol;    High-mobility group box 1;    Interleukin-6;    Interleukin-8;    Tumor necrosis factor-α;   

DOI  :  10.1186/s12871-017-0441-0

 received in 2017-07-24, accepted in 2017-10-19,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundStudies have found that propofol can inhibit endotoxin-induced monocyte-macrophages to produce various inflammatory factors. This study is to disclose whether the propofol affects the expression of high-mobility group box 1 (HMGB1) in lipopolysaccharides (LPS)-stimulated RAW 264.7 cells and the release of interleukin-6 (IL-6), 8 (IL-8) and tumor necrosis factor-α (TNF-α).MethodsRAW 264.7 cells were divided into four groups for intervention. After culturing for 16 h, the cells and culture supernatants were collected. The expression of HMGB1 in RAW 264.7 cells was detected by Western blot. The levels of IL-6, IL-8 and TNF-α in supernatants of cells were determined by enzyme-linked immunosorbent assay (ELISA).ResultsStimulation of LPS increased the expression of HMGB1 and promoted the release of IL-6, IL-8 and TNF-α in supernatants of RAW 264.7 cells (p < 0.05); however, propofol down-regulated the expression of LPS-stimulated HMGB1 and reduced the LPS-stimulated releases of IL-6, IL-8 and TNF-α in supernatants of RAW 264.7 cells (p < 0.05). Moreover, the releases of IL-6, IL-8 and TNF-α intimately correlated with the expression of HMGB1 in this process (p < 0.05).ConclusionPropofol inhibited the releases of IL-6, IL-8 and TNF-α in LPS-stimulated RAW 264.7 cells, and the levels of IL-6, IL-8 and TNF-α intimately correlated with the expression of HMGB1, which indicating that propofol may prevent inflammatory responses through reducing the releases of these cytokines and inflammatory mediators.

【 授权许可】

CC BY   
© The Author(s). 2017