Enhanced photodynamic efficacy and efficient delivery of Rose Bengal using nanostructured poly(amidoamine) dendrimers: potential application in photodynamic therapy of cancer

Cancer Nanotechnology

Original Paper

Kadarkaraithangam Jeyasubramanian¹  Sang-Jae Kim²  Krishnamoorthy Karthikeyan³  Anish Babu⁴  Ramachandran Murugesan⁴ 

[1]Department of Nanoscience and Technology, Mepco Schlenk Engineering College, Sivakasi, Tamilnadu, India

[2]Nanomaterials and System Laboratory, Department of Mechanical Engineering, Jeju National University, Jeju, South Korea

[3]Nanomaterials and System Laboratory, Department of Mechanical Engineering, Jeju National University, Jeju, South Korea

[4]Department of Nanoscience and Technology, Mepco Schlenk Engineering College, Sivakasi, Tamilnadu, India

[5]School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamilnadu, India

关键词: Photodynamic therapy;    Dendrimer;    Drug delivery;    Phototoxicity;    Dark toxicity;    Reactive oxygen species;   

DOI  :  10.1007/s12645-011-0019-3

 received in 2011-06-28, accepted in 2011-07-27,  发布年份 2011

来源: Springer

【 摘 要 】

Photodynamic therapy (PDT) is a promising treatment methodology whereby diseased cells and tissues are destroyed by reactive oxygen species (ROS) by using a combination of light and photosensitizers (PS). The medical application of Rose Bengal (RB), photosensitizer with very good ROS generation capability, is limited due to its intrinsic toxicity and insufficient lipophilicity. In this report, we evaluate the potential of polyamidoamine (PAMAM) dendrimers in delivering RB and its phototoxic efficiency towards a model cancer cell line. The spherical, nanoscaled dendrimers could efficiently encapsulate RB and showed characteristic spectral responses. The controlled release property of dendrimer–RB formulation was clearly evident from the in vitro drug release study. ROS generation was confirmed in dendrimer–RB system upon white light illumination. Photosensitization of Dalton’s Lymphoma Ascite (DLA) cells incubated with dendrimer–RB formulation caused remarkable photocytotoxicity. Importantly, the use of dendrimer-based delivery system reduced the dark toxicity of RB.

【 授权许可】

Unknown   
© Springer-Verlag 2011