Membrane roughness as a sensitive parameter reflecting the status of neuronal cells in response to chemical and nanoparticle treatments

Journal of Nanobiotechnology

Methodology

Yun-Ru Chen¹  Chih-Cheng Chen²  Lan-Ling Jang³  Huei-Jyuan Pan³  Chia-Wei Lee³  Chau-Hwang Lee⁴ 

[1] Genomics Research Center, Academia Sinica, 11529, Taipei, Taiwan;Institute of Biomedical Sciences, Academia Sinica, 11529, Taipei, Taiwan;Research Center for Applied Sciences, Academia Sinica, 128 Sec. 2, Academia Road, Nankang, 11529, Taipei, Taiwan;Research Center for Applied Sciences, Academia Sinica, 128 Sec. 2, Academia Road, Nankang, 11529, Taipei, Taiwan;Institute of Biophotonics, National Yang-Ming University, 11221, Taipei, Taiwan;Department of Physics, National Taiwan University, 10617, Taipei, Taiwan;

关键词: Optical profilometry;    Neuroblastoma cell;    Paclitaxel;    Charged nanoparticle;   

DOI  :  10.1186/s12951-016-0161-5

 received in 2015-10-29, accepted in 2016-01-20,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundCell membranes exhibit abundant types of responses to external stimulations. Intuitively, membrane topography should be sensitive to changes of physical or chemical factors in the microenvironment. We employed the non-interferometric wide-field optical profilometry (NIWOP) technique to quantify the membrane roughness of living neuroblastoma cells under various treatments that could change the mechanical properties of the cells.ResultsThe membrane roughness was reduced as the neuroblastoma cell was treated with paclitaxel, which increases cellular stiffness by translocating microtubules toward the cell membranes. The treatment of positively charged gold nanoparticles (AuNPs) showed a similar effect. In contrast, the negatively charged AuNPs did not cause significant changes of the membrane roughness. We also checked the membrane roughness of fixed cells by using scanning electron microscopy (SEM) and confirmed that the membrane roughness could be regarded as a parameter reflecting cellular mechanical properties. Finally, we monitored the temporal variations of the membrane roughness under the treatment with a hypertonic solution (75 mM sucrose in the culture medium). The membrane roughness was increased within 1 h but returned to the original level after 2 h.ConclusionsThe results in the present study suggest that the optical measurement on membrane roughness can be regarded as a label-free method to monitor the changes in cell mechanical properties or binding properties of nanoparticles on cell surface. Because the cells were left untouched during the measurement, further tests about cell viability or drug efficacy can be done on the same specimen. Membrane roughness could thus provide a quick screening for new chemical or physical treatments on neuronal cells.

【 授权许可】

CC BY   
© Lee et al. 2016