A PKCβ isoform mediates phorbol ester‐induced activation of Erk1/2 and expression of neuronal differentiation genes in neuroblastoma cells

FEBS Letters

Trollér, Ulrika¹  Larsson, Christer¹  Svensson, Karin¹  Zeidman, Ruth¹ 

[1] Lund University, Molecular Medicine, Entrance 78, 3rd floor, Malmö University Hospital, 205 02 Malmö, Sweden

关键词: Growth-associated protein 43;    Mitogen-activated protein kinase;    Neuroblastoma cell;    Neuronal differentiation;    Neuropeptide Y;    Protein kinase C;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    GAP-43;    growth-associated protein 43;    NPY;    neuropeptide Y;    PKC;    protein kinase C;    TPA;    12-O-tetradecanoylphorbol 13-acetate;   

DOI  :  10.1016/S0014-5793(01)03043-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein kinase C (PKC) activation induces neuronal differentiation of SH-SY5Y neuroblastoma cells. This study examines the role of PKCβ isoforms in this process. The PKCβ-specific inhibitor LY379196 had no effect on 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced neurite outgrowth from SH-SY5Y neuroblastoma cells. On the other hand, PKCβ inhibition suppressed the TPA-stimulated increase in neuropeptide Y mRNA, activation of neuropeptide Y gene promoter elements, and phosphorylation of Erk1/2. The TPA-induced increase in neuropeptide Y expression was also inhibited by the MEK inhibitor PD98059. These data indicate that activation of a PKCβ isoform, through a pathway involving Erk1/2, leads to increased expression of neuronal differentiation genes in neuroblastoma cells.

【 授权许可】

Unknown