Gene signatures in wound tissue as evidenced by molecular profiling in the chick embryo model

BMC Genomics

Research Article

Witold W Kilarski¹  Andreas Bikfalvi¹  Fabienne Soulet¹  Roy Bicknell²  John Herbert²  Philipp Antczak²  Francesco Falciani² 

[1] INSERM, U920, 3340, Talence, France;Université Bordeaux I, 33 405, Talence, France;Institute of Biomedical Research, University of Birmingham, B15 2TT, Birmingham, UK;

关键词: Granulation Tissue;    Ingenuity Pathway Analysis;    Granulation Tissue Formation;    Chicken Gene;    Endothelial Cell Expression;   

DOI  :  10.1186/1471-2164-11-495

 received in 2010-02-05, accepted in 2010-09-14,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundModern functional genomic approaches may help to better understand the molecular events involved in tissue morphogenesis and to identify molecular signatures and pathways. We have recently applied transcriptomic profiling to evidence molecular signatures in the development of the normal chicken chorioallantoic membrane (CAM) and in tumor engrafted on the CAM. We have now extended our studies by performing a transcriptome analysis in the "wound model" of the chicken CAM, which is another relevant model of tissue morphogenesis.ResultsTo induce granulation tissue (GT) formation, we performed wounding of the chicken CAM and compared gene expression to normal CAM at the same stage of development. Matched control samples from the same individual were used. We observed a total of 282 genes up-regulated and 44 genes down-regulated assuming a false-discovery rate at 5% and a fold change > 2. Furthermore, bioinformatics analysis lead to the identification of several categories that are associated to organismal injury, tissue morphology, cellular movement, inflammatory disease, development and immune system. Endothelial cell data filtering leads to the identification of several new genes with an endothelial cell signature.ConclusionsThe chick chorioallantoic wound model allows the identification of gene signatures and pathways involved in GT formation and neoangiogenesis. This may constitute a fertile ground for further studies.

【 授权许可】

Unknown   
© Soulet et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.