Jejunal gene expression patterns correlate with severity of systemic infection in chicken

BMC Proceedings

Proceedings

Johanna MJ Rebel¹  Dirkjan Schokker²  Mari A Smits² 

[1] Central Veterinary Institute, P.O. Box 65, 8200 AB, Wageningen UR, Lelystad, The Netherlands;Wageningen UR Livestock Research, Animal Breeding and Genomics Centre, P.O. Box 65, 8200 AB, Lelystad, The Netherlands;

关键词: Morphological Process;    Junction Adhesion Molecule;    Functional Annotation Cluster;    Chicken Gene;    Intestinal Development;   

DOI  :  10.1186/1753-6561-5-S4-S4

来源: Springer

【 摘 要 】

BackgroundNot much is known about the effect of Salmonella enteritidis on changes in the developmental processes occurring in the intestine of young chicken. Therefore we investigated the correlation of intestinal gene expression patterns with the severity of systemic Salmonella infections.MethodsThe number of Salmonella colony forming units (CFUs) in the liver of infected chicken were plotted against the average intestinal expression profiles of previously identified gene expression clusters. The functional properties of all the genes taken together present in 3 clusters exhibiting positive correlation at early time-points were compared with the functional properties of the genes displaying antagonistic correlations in 1 cluster. The top 5 ranking functional groups were analysed in further detail.ResultsThree clusters showed gene expression profiles which were positively correlated with the severity of systemic disease as measured by the number of Salmonella colony forming units in the liver. In these clusters, genes involved in morphological processes were predominantly present. One cluster had a profile that was negatively correlated with the severity of systemic disease, as measured by numbers of CFUs in the liver. The genes in the latter cluster were mostly involved in cell turn-over and metabolism.ConclusionsIn the developing jejunum of young chicken, both stimulatory and inhibitory gene expression mechanisms are correlated with the severity of systemic Salmonella infections.

【 授权许可】

CC BY   
© Schokker et al; licensee BioMed Central Ltd. 2011