期刊论文详细信息
Journal of Nanobiotechnology
Interaction of silver nanoparticles with algae and fish cells: a side by side comparison
Research
Marc J-F Suter1  Smitha Pillai1  Renata Behra1  Laura Sigg2  Xiaomei Li3  Yang Yue4  Kristin Schirmer5 
[1] Department of Environmental Toxicology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600, Dübendorf, Switzerland;Department of Environmental Systems Science (D-USYS), ETH-Zürich, 8092, Zürich, Switzerland;Department of Environmental Toxicology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600, Dübendorf, Switzerland;Department of Environmental Systems Science (D-USYS), ETH-Zürich, 8092, Zürich, Switzerland;Wattstrasse 13a, 8307, Effretikon, Switzerland;Department of Environmental Toxicology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600, Dübendorf, Switzerland;School of Architecture, Civil and Environmental Engineering, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland;Department of Environmental Toxicology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600, Dübendorf, Switzerland;School of Architecture, Civil and Environmental Engineering, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland;Department of Basic Sciences and Aquatic Medicine, Norwegian University of Life Sciences (NMBU), 0454, Oslo, Norway;Department of Environmental Toxicology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600, Dübendorf, Switzerland;School of Architecture, Civil and Environmental Engineering, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland;Department of Environmental Systems Science (D-USYS), ETH-Zürich, 8092, Zürich, Switzerland;
关键词: AgNP;    Euglena gracilis;    RTgill-W1 cell line;    Nanoparticle uptake;    Nanoparticle toxicity;    Nanoparticle-protein interactions;   
DOI  :  10.1186/s12951-017-0254-9
 received in 2016-09-02, accepted in 2017-02-22,  发布年份 2017
来源: Springer
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【 摘 要 】

BackgroundSilver nanoparticles (AgNP) are widely applied and can, upon use, be released into the aquatic environment. This raises concerns about potential impacts of AgNP on aquatic organisms. We here present a side by side comparison of the interaction of AgNP with two contrasting cell types: algal cells, using the algae Euglena gracilis as model, and fish cells, a cell line originating from rainbow trout (Oncorhynchus mykiss) gill (RTgill-W1). The comparison is based on the AgNP behavior in exposure media, toxicity, uptake and interaction with proteins.Results(1) The composition of exposure media affected AgNP behavior and toxicity to algae and fish cells. (2) The toxicity of AgNP to algae was mediated by dissolved silver while nanoparticle specific effects in addition to dissolved silver contributed to the toxicity of AgNP to fish cells. (3) AgNP did not enter into algal cells; they only adsorbed onto the cell surface. In contrast, AgNP were taken up by fish cells via endocytic pathways. (4) AgNP can bind to both extracellular and intracellular proteins and inhibit enzyme activity.ConclusionOur results showed that fish cells take up AgNP in contrast to algal cells, where AgNP sorbed onto the cell surface, which indicates that the cell wall of algae is a barrier to particle uptake. This particle behaviour results in different responses to AgNP exposure in algae and fish cells. Yet, proteins from both cell types can be affected by AgNP exposure: for algae, extracellular proteins secreted from cells for, e.g., nutrient acquisition. For fish cells, intracellular and/or membrane-bound proteins, such as the Na+/K+-ATPase, are susceptible to AgNP binding and functional impairment.

【 授权许可】

CC BY   
© The Author(s) 2017

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