Evaluating the effect of assay preparation on the uptake of gold nanoparticles by RAW264.7 cells

Journal of Nanobiotechnology

Katherine M Tyner¹  Simona Bancos¹ 

[1] Center for Drug Evaluation and Research, Food and Drug Administration, Building 51 Room 4159, 10903 New Hampshire Ave., Silver Spring 20993, MD, USA

关键词: Macrophage;    Nanoparticle dosimetry;    In vitro assay;    Nanoparticle uptake;    Gold nanoparticle;   

Others  :  1139419
DOI  :  10.1186/s12951-014-0045-5

 received in 2014-08-11, accepted in 2014-10-19,  发布年份 2014

【 摘 要 】

Background

Cell culture conditions can greatly influence the results of nanoparticle (NP) uptake assays. In this study, 10 nm gold nanoparticles (AuNPs) and RAW 264.7 macrophages were used as a model system, while instrumental neutron activation analysis (NAA) was used as the elemental analysis technique to determine AuNP levels produced by the various culturing conditions. Static plate-based and insert-based culture conditions were compared with a dynamic suspension culture to evaluate the conditions’ effect on the rate and extent of AuNP uptake.

Results

The results indicate that a dynamic culturing condition allows for the greatest NP uptake (approximately 3-5 times over the adherent conditions), whereas the plate-based assays have the initial highest rate of NP incorporation.

Conclusions

These data highlight the importance of judiciously choosing the assay conditions prior to evaluating NP uptake.

【 授权许可】

   
2014 Bancos and Tyner; licensee BioMed Central Ltd.

【 图 表 】

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