Apoptosis inhibition or inflammation: the role of NAIP protein expression in Hodgkin and non-Hodgkin lymphomas compared to non-neoplastic lymph node

Journal of Inflammation

Research

Mahdad Esmaeili¹  Safoura Mazrouei²  Mansoor Salehi³  Amin Ziaei⁴  Azar Baradaran⁵  Amir Pouya Tanhaee⁶  Kianoosh Keyhanian⁷ 

[1] Dept of Biomedical Engineering, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran;Dept of Genetics and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran;Faculty of Medicine, Friedrich Schiller University of Jena, Jena, Germany;Dept of Genetics and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran;Medical Genetics Center of Genome, No 208, Shariati St. (West), Isfahan, Iran;Dept of Genetics and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran;Medical Student Research Center, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran;Dept of Pathology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran;Medical Student Research Center, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran;Ottawa Hospital Research Institute, Ottawa, Ontario, Canada;

关键词: Inhibitor of apoptosis protein;    NAIP/BIRC1;    Inflammatory caspases;    Inflammasome;    Hodgkin lymphoma;    Non-Hodgkin lymphoma;    Semi-quantitative immuno-flourecent staining;   

DOI  :  10.1186/1476-9255-9-4

 received in 2011-07-29, accepted in 2012-02-23,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundInhibitors of Apoptosis (IAP) family play a critical role in apoptosis and inflammatory response. Neuronal Apoptosis Inhibitory Protein (NAIP), as a member of both IAPs and NLR families (NOD-Like Receptor), is a unique IAP harboring NOD (Nucleotide Oligomerization Domain) and LLR (Leucine Rich Repeat) motifs. Considering these motifs in NAIP, it has been suggested that the main function of NAIP is distinct from other members of IAPs. As a member of NLR, NAIP mediates the assembly of 'Inflammasome' for inflammatory caspase activation. Pathologic expression of NAIP has been reported not only in some infectious and inflammatory diseases but also in some malignancies. However, there is no report to elucidate NAIP expression in lymphomatic malignancies.MethodsIn this study, we examined NAIP protein expression in 101 Formalin-Fixed Paraffin-Embedded blocks including samples from 39 Hodgkin Lymphoma and 23 Non Hodgkin Lymphoma cases in comparison with 39 control samples (30 normal and 9 Reactive Lymphoid Hyperplasia (RLH) lymph nodes) using semi-quantitative immuno-flourecent Staining.ResultsNAIP expression was not statistically different in lymphoma samples neither in HL nor in NHL cases comparing to normal samples. However, we evaluated NAIP expression in normal and RLH lymph nodes. Surprisingly, we have found a statistically significant-difference between the NAIP expression in RLH (M.R of NAIP/GAPDH expression = 0.6365 ± 0.017) and normal lymph node samples (M.R of NAIP/GAPDH expression = 0.5882 ± 0.047) (P < 0.01).ConclusionsThese findings show that the regulation of apoptosis could not be the main function of NAIP in the cell, so the pathologic expression of NAIP is not involved in lymphoma. But, we concluded that the over expression of NAIP has more effective role in the inflammatory response. Also, this study clarifies the NAIP expression level in lymphoma which is required for IAPs profiling in order to be used in potential translational applications of IAPs.

【 授权许可】

Unknown   
© Mazrouei et al; BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.