The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniaeand comparison with four other species specific PCR assays

BMC Infectious Diseases

Research Article

Rita Verhelst¹  Elife Alkan¹  Mario Vaneechoutte¹  Nabil Abdullah El Aila¹  Bart Saerens¹  Pieter Deschaght¹  Thierry De Baere²  Tarja Kaijalainen³  Stefan Emler⁴ 

[1] Laboratory Bacteriology Research, Department of Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium;Laboratory Bacteriology Research, Department of Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium;Scientific Institute of Public Health, Brussels, Belgium;National Reference Laboratory for Pneumococcus, National Institute for Health and Welfare (THL), Oulu, Finland;SmartGene, Zug, Switzerland;

关键词: Chronic Obstructive Pulmonary Disease;    Moraxella Catarrhalis;    Streptococcus Agalactiae;    Viridans Streptococcus;    Streptococcal Strain;   

DOI  :  10.1186/1471-2334-10-104

 received in 2009-11-24, accepted in 2010-04-29,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundStreptococcus pneumoniae is one of the most frequently encountered pathogens in humans but its differentiation from closely related but less pathogenic streptococci remains a challenge.MethodsThis report describes a newly-developed PCR assay (Spne-PCR), amplifying a 217 bp product of the 16S rRNA gene of S. pneumoniae, and its performance compared to other genotypic and phenotypic tests.ResultsThe new PCR assay designed in this study, proved to be specific at 57°C for S. pneumoniae, not amplifying S. pseudopneumoniae or any other streptococcal strain or any strains from other upper airway pathogenic species. PCR assays (psaA, LytA, ply, spn9802-PCR) were previously described for the specific amplification of S. pneumoniae, but psaA-PCR was the only one found not to cross-react with S. pseudopneumoniae.ConclusionSpne-PCR, developed for this study, and psaA-PCR were the only two assays which did not mis-identify S. pseudopneumoniae as S. pneumoniae. Four other PCR assays and the AccuProbe assay were unable to distinguish between these species.

【 授权许可】

Unknown   
© El Aila et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.