Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination

BMC Plant Biology

Research Article

Jinggong Guo¹  Jingliang Tang¹  Shuang Han¹  Yuchen Miao¹  Baodi Bi¹ 

[1] Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, 475001, Kaifeng, China;

关键词: Sinapic acid;    Sinapic acid esters;    Abscisic acid homeostasis;    Seed germination;    Arabidopsis thaliana;   

DOI  :  10.1186/s12870-017-1048-9

 received in 2016-12-18, accepted in 2017-05-25,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundSinapic acid and its esters have broad functions in different stages of seed germination and plant development and are thought to play a role in protecting against ultraviolet irradiation. To better understand the interactions between sinapic acid esters and seed germination processes in response to various stresses, we analyzed the role of the plant hormone abscisic acid (ABA) in the regulation of sinapic acid esters involved in seed germination and early seedling growth.ResultsWe found that exogenous sinapic acid promotes seed germination in a dose-dependent manner in Arabidopsis thaliana. High-performance liquid chromatography mass spectrometry analysis showed that exogenous sinapic acid increased the sinapoylcholine content of imbibed seeds. Furthermore, sinapic acid affected ABA catabolism, resulting in reduced ABA levels and increased levels of the ABA-glucose ester. Using mutants deficient in the synthesis of sinapate esters, we showed that the germination of mutant sinapoylglucose accumulator 2 (sng2) and bright trichomes 1 (brt1) seeds was more sensitive to ABA than the wild-type. Moreover, Arabidopsis mutants deficient in either abscisic acid deficient 2 (ABA2) or abscisic acid insensitive 3 (ABI3) displayed increased expression of the sinapoylglucose:choline sinapoyltransferase (SCT) and sinapoylcholine esterase (SCE) genes with sinapic acid treatment. This treatment also affected the accumulation of sinapoylcholine and free choline during seed germination.ConclusionsWe demonstrated that sinapoylcholine, which constitutes the major phenolic component in seeds among various minor sinapate esters, affected ABA homeostasis during seed germination and early seedling growth in Arabidopsis. Our findings provide insights into the role of sinapic acid and its esters in regulating ABA-mediated inhibition of Arabidopsis seed germination in response to drought stress.

【 授权许可】

CC BY   
© The Author(s). 2017