Journal of Inflammation | |
Crude extract of hydatid laminated layer from Echinococcus granulosus cyst attenuates mucosal intestinal damage and inflammatory responses in Dextran Sulfate Sodium induced colitis in mice | |
Research | |
Hayet Rafa1  Imene Soufli1  Ryma Toumi1  Lila Khelifi1  Manel Amri1  Chafia Touil-Boukoffa1  Moussa Labsi1  Ferdinando Nicoletti2  | |
[1] Department of Biology, Laboratory of Cellular and Molecular Biology, University of Sciences and Technology Houari Boumediene USTHB, Algiers, Algeria;Department of Biomedical Sciences, University of Catania, Catania, Italy; | |
关键词: Inflammatory bowel disease (IBD); Experimental colitis; Nitric oxide (NO); Inducible nitric oxide synthase (iNOS); Nuclear factor-κB; Interferon (IFN)-γ; tumor necrosis factor (TNF)-α; helminth; hydatid laminated layer; Echinococcus granulosus; | |
DOI : 10.1186/s12950-015-0063-6 | |
received in 2014-06-29, accepted in 2015-02-24, 发布年份 2015 | |
来源: Springer | |
【 摘 要 】
BackgroundInflammatory bowel disease is an immunologically mediated disease. Notably, it is less common in countries where there is a greater risk of exposure to helminths. In our study, we examined the modulatory effect of the laminated layer extracted from the cyst wall of a helminth parasite, Echinococcus granulosus, on dextran sulfate sodium (DSS)-induced colitis in mice.MethodsAn acute colitis was induced in BALB/c mice using 2.5% w/v DSS in drinking water. The crude extract of E. granulosus laminated layer was injected intraperitoneally daily, starting 3 days before colitis induction. The Disease Activity Index was monitored daily, colon length and weight were measured and histological scores were evaluated. Nitric oxide (NO) and cytokine levels (interferon γ (IFN-γ), tumor necrosis factor α (TNF-α) and interleukin 10 (IL-10)) were assessed by enzyme-linked immunosorbent assay. In addition, the colonic expression of inducible nitric oxide synthase (iNOS) and nuclear factor-κB (NF-κB) was examined. Statistical analyses were performed by one-way analysis of variance and the survival rate was analyzed by the long rank test.ResultsHydatid laminated layer pretreatment significantly improved the clinical symptoms and histological scores (*** p < 0.01) observed during DSS-induced colitis and maintained mucus production by goblet cells. Furthermore, treatment with hydatid laminated layer caused a significant decrease in NO, IFN-γ (** p < 0.01) and TNF-α production (* p < 0.05) and an increase in IL-10 production. These results were associated with localized downregulation of iNOS and NF-κB expression.ConclusionsOur results demonstrate the potent anti-inflammatory effects of hydatid laminated layer. Furthermore, preventive treatment with the laminated layer played a beneficial role in maintaining the integrity of the intestinal mucosal barrier against DSS-induced injury.
【 授权许可】
Unknown
© Soufli et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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