Crude extract of hydatid laminated layer from Echinococcus granulosus cyst attenuates mucosal intestinal damage and inflammatory responses in Dextran Sulfate Sodium induced colitis in mice

Journal of Inflammation

Research

Hayet Rafa¹  Imene Soufli¹  Ryma Toumi¹  Lila Khelifi¹  Manel Amri¹  Chafia Touil-Boukoffa¹  Moussa Labsi¹  Ferdinando Nicoletti² 

[1] Department of Biology, Laboratory of Cellular and Molecular Biology, University of Sciences and Technology Houari Boumediene USTHB, Algiers, Algeria;Department of Biomedical Sciences, University of Catania, Catania, Italy;

关键词: Inflammatory bowel disease (IBD);    Experimental colitis;    Nitric oxide (NO);    Inducible nitric oxide synthase (iNOS);    Nuclear factor-κB;    Interferon (IFN)-γ;    tumor necrosis factor (TNF)-α;    helminth;    hydatid laminated layer;    Echinococcus granulosus;   

DOI  :  10.1186/s12950-015-0063-6

 received in 2014-06-29, accepted in 2015-02-24,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundInflammatory bowel disease is an immunologically mediated disease. Notably, it is less common in countries where there is a greater risk of exposure to helminths. In our study, we examined the modulatory effect of the laminated layer extracted from the cyst wall of a helminth parasite, Echinococcus granulosus, on dextran sulfate sodium (DSS)-induced colitis in mice.MethodsAn acute colitis was induced in BALB/c mice using 2.5% w/v DSS in drinking water. The crude extract of E. granulosus laminated layer was injected intraperitoneally daily, starting 3 days before colitis induction. The Disease Activity Index was monitored daily, colon length and weight were measured and histological scores were evaluated. Nitric oxide (NO) and cytokine levels (interferon γ (IFN-γ), tumor necrosis factor α (TNF-α) and interleukin 10 (IL-10)) were assessed by enzyme-linked immunosorbent assay. In addition, the colonic expression of inducible nitric oxide synthase (iNOS) and nuclear factor-κB (NF-κB) was examined. Statistical analyses were performed by one-way analysis of variance and the survival rate was analyzed by the long rank test.ResultsHydatid laminated layer pretreatment significantly improved the clinical symptoms and histological scores (*** p < 0.01) observed during DSS-induced colitis and maintained mucus production by goblet cells. Furthermore, treatment with hydatid laminated layer caused a significant decrease in NO, IFN-γ (** p < 0.01) and TNF-α production (* p < 0.05) and an increase in IL-10 production. These results were associated with localized downregulation of iNOS and NF-κB expression.ConclusionsOur results demonstrate the potent anti-inflammatory effects of hydatid laminated layer. Furthermore, preventive treatment with the laminated layer played a beneficial role in maintaining the integrity of the intestinal mucosal barrier against DSS-induced injury.

【 授权许可】

Unknown   
© Soufli et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.