The flesh ethanolic extract of Hylocereus polyrhizus exerts anti-inflammatory effects and prevents murine colitis

卷:35

Macias-Ceja, Dulce C. ; Cosin-Roger, Jesus ; Ortiz-Masia, Dolores ; Salvador, Pedro ; Hernandez, Carlos ; Calatayud, Sara ; Esplugues, Juan V. ; Barrachina, Maria D.

Univ Valencia

关键词: Inflammation;    Inflammatory bowel disease;    Cytokines;    NF-kappa B;    Experimental colitis;    Hylocereus polyrhizus;   

DOI  :  10.1016/j.clnu.2016.02.013

学科分类：食品科学和技术

【 摘 要 】

Background & aims: IBD is a chronic disorder of the gastrointestinal tract characterized by mucosal inflammation and epithelial damage. Biologic therapy has significantly improved the course of the disease but there are still a high percentage of patients that do not respond to current therapies. We aim to determine the effects of the flesh ethanolic extract of Hylocereus polyrhizus (EH) in a mice model of colitis induced by TNBS. Methods: Balb/c mice received TNBS (175 mg/kg, 100 mu l, i.r.) and six and thirty hours later were administered with EH (1 g/kg, i.p.). Mice were weighted daily and after sacrificing (2 and 4 days after TNBS) we analyzed mucosal histology, myeloperoxidase activity (MPO), the expression of pro inflammatory molecules (qPCR) and NF-kappa B and I kappa beta-alpha protein levels. The chemical characterization of the EH was determined by LC-MS/MS. Results: The administration of EH to TNBS-treated mice prevented (P < 0.05) the loss of body weight and significantly reduced in the colon: a) histological damage score, b) MPO enzymatic activity c) the expression of pro-inflammatory molecules and d) I kappa beta-alpha degradation and nuclear NF-kappa beta protein levels. The LC-MS analysis detected metabolites such as polyphenols and fatty acids. Conclusion: Systemic administration of the ethanolic extract of H. polyrhizus exerts an anti-inflammatory effect and prevents murine colitis induced by TNBS. (C) 2016 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.