卷:35 | |
The flesh ethanolic extract of Hylocereus polyrhizus exerts anti-inflammatory effects and prevents murine colitis | |
Macias-Ceja, Dulce C. ; Cosin-Roger, Jesus ; Ortiz-Masia, Dolores ; Salvador, Pedro ; Hernandez, Carlos ; Calatayud, Sara ; Esplugues, Juan V. ; Barrachina, Maria D. | |
Univ Valencia | |
关键词: Inflammation; Inflammatory bowel disease; Cytokines; NF-kappa B; Experimental colitis; Hylocereus polyrhizus; | |
DOI : 10.1016/j.clnu.2016.02.013 | |
学科分类:食品科学和技术 | |
【 摘 要 】
Background & aims: IBD is a chronic disorder of the gastrointestinal tract characterized by mucosal inflammation and epithelial damage. Biologic therapy has significantly improved the course of the disease but there are still a high percentage of patients that do not respond to current therapies. We aim to determine the effects of the flesh ethanolic extract of Hylocereus polyrhizus (EH) in a mice model of colitis induced by TNBS. Methods: Balb/c mice received TNBS (175 mg/kg, 100 mu l, i.r.) and six and thirty hours later were administered with EH (1 g/kg, i.p.). Mice were weighted daily and after sacrificing (2 and 4 days after TNBS) we analyzed mucosal histology, myeloperoxidase activity (MPO), the expression of pro inflammatory molecules (qPCR) and NF-kappa B and I kappa beta-alpha protein levels. The chemical characterization of the EH was determined by LC-MS/MS. Results: The administration of EH to TNBS-treated mice prevented (P < 0.05) the loss of body weight and significantly reduced in the colon: a) histological damage score, b) MPO enzymatic activity c) the expression of pro-inflammatory molecules and d) I kappa beta-alpha degradation and nuclear NF-kappa beta protein levels. The LC-MS analysis detected metabolites such as polyphenols and fatty acids. Conclusion: Systemic administration of the ethanolic extract of H. polyrhizus exerts an anti-inflammatory effect and prevents murine colitis induced by TNBS. (C) 2016 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
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