Production of cecropin A antimicrobial peptide in rice seed endosperm

BMC Plant Biology

Research Article

Emmanuel Guiderdoni¹  Delphine Mieulet¹  Mireia Bundó²  María Coca²  Blanca San Segundo²  Sonia Campo²  Laura Montesinos³  Esther Badosa³  Emilio Montesinos³  Esther Izquierdo⁴  Michel Rossignol⁴ 

[1] Centre de Coopération Internationale en Recherche Agronomique pour le Développement, UMR AGAP, Genetic Improvement and Adaptation of Mediterranean and Tropical Plants, CIRAD, 34398, Montpellier, Cedex 5, France;Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Edifici CRAG, Campus de la UAB, 08193, Bellaterra, Barcelona, Spain;Institute of Food and Agricultural Technology-CIDSAV-XaRTA, University of Girona, 17071, Girona, Spain;Mass Spectrometry Proteomics Platform-MSPP, Laboratoire de Protéomique Fonctionnelle, INRA, 34060, Montpellier, Cedex 1, France;

关键词: Rice;    Antimicrobial peptides;    Cecropin A;    Endosperm;    Protein bodies;    Pathogen resistance;    Fusarium verticillioides;    Dickeya dadantii;    Oryza sativa;   

DOI  :  10.1186/1471-2229-14-102

 received in 2013-10-04, accepted in 2014-04-14,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundCecropin A is a natural antimicrobial peptide that exhibits rapid, potent and long-lasting lytic activity against a broad spectrum of pathogens, thus having great biotechnological potential. Here, we report a system for producing bioactive cecropin A in rice seeds.ResultsTransgenic rice plants expressing a codon-optimized synthetic cecropin A gene drived by an endosperm-specific promoter, either the glutelin B1 or glutelin B4 promoter, were generated. The signal peptide sequence from either the glutelin B1 or the glutelin B4 were N-terminally fused to the coding sequence of the cecropin A. We also studied whether the presence of the KDEL endoplasmic reticulum retention signal at the C-terminal has an effect on cecropin A subcellular localization and accumulation. The transgenic rice plants showed stable transgene integration and inheritance. We show that cecropin A accumulates in protein storage bodies in the rice endosperm, particularly in type II protein bodies, supporting that the glutelin N-terminal signal peptides play a crucial role in directing the cecropin A to this organelle, independently of being tagged with the KDEL endoplasmic reticulum retention signal. The production of cecropin A in transgenic rice seeds did not affect seed viability or seedling growth. Furthermore, transgenic cecropin A seeds exhibited resistance to infection by fungal and bacterial pathogens (Fusarium verticillioides and Dickeya dadantii, respectively) indicating that the in planta-produced cecropin A is biologically active.ConclusionsRice seeds can sustain bioactive cecropin A production and accumulation in protein bodies. The system might benefit the production of this antimicrobial agent for subsequent applications in crop protection and food preservation.

【 授权许可】

Unknown   
© Bundó et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.