Improved bioenergy value of residual rice straw by increased lipid levels from upregulation of fatty acid biosynthesis

Biotechnology for Biofuels and Bioproducts

Research

Simon Isaksson¹  Anna Schnürer¹  Selcuk Aslan²  Chuanxin Sun²  Folke Sitbon²  Jia Hu²  Yunkai Jin²  Lu Jin³  Jun Su⁴  Per Hofvander⁵  Chunlin Liu⁶  Feng Wang⁷  Yan Lin⁷ 

[1] Department of Molecular Sciences, Swedish University of Agricultural Sciences, P. O. Box 7015, 750 07, Uppsala, Sweden;Department of Plant Biology, The Linnean Centre for Plant Biology, Swedish University of Agricultural Sciences, P. O. Box 7080, 75007, Uppsala, Sweden;Department of Plant Biology, The Linnean Centre for Plant Biology, Swedish University of Agricultural Sciences, P. O. Box 7080, 75007, Uppsala, Sweden;Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, 410128, Changsha, China;Department of Plant Biology, The Linnean Centre for Plant Biology, Swedish University of Agricultural Sciences, P. O. Box 7080, 75007, Uppsala, Sweden;Institute of Biotechnology, Fujian Academy of Agricultural Sciences, 350003, Fuzhou, China;Department of Plant Breeding, Swedish University of Agricultural Sciences, P.O. Box 190, 23422, Lomma, Sweden;Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, 410128, Changsha, China;Institute of Biotechnology, Fujian Academy of Agricultural Sciences, 350003, Fuzhou, China;

关键词: WRINKLED1;    Triacylglycerol (TAG);    Rice;    Endosperm;    Bioenergy;    Anaerobic digestion;    Rice straw;   

DOI  :  10.1186/s13068-023-02342-y

 received in 2022-09-16, accepted in 2023-05-13,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundRice (Oryza sativa) straw is a common waste product that represents a considerable amount of bound energy. This energy can be used for biogas production, but the rate and level of methane produced from rice straw is still low. To investigate the potential for an increased biogas production from rice straw, we have here utilized WRINKLED1 (WRI1), a plant AP2/ERF transcription factor, to increase triacylglycerol (TAG) biosynthesis in rice plants. Two forms of Arabidopsis thaliana WRI1 were evaluated by transient expression and stable transformation of rice plants, and transgenic plants were analyzed both for TAG levels and biogas production from straw.ResultsBoth full-length AtWRI1, and a truncated form lacking the initial 141 amino acids (including the N-terminal AP2 domain), increased fatty acid and TAG levels in vegetative and reproductive tissues of Indica rice. The stimulatory effect of the truncated AtWRI1 was significantly lower than that of the full-length protein, suggesting a role for the deleted AP2 domain in WRI1 activity. Full-length AtWRI1 increased TAG levels also in Japonica rice, indicating a conserved effect of WRI1 in rice lipid biosynthesis. The bio-methane production from rice straw was 20% higher in transformants than in the wild type. Moreover, a higher producing rate and final yield of methane was obtained for rice straw compared with rice husks, suggesting positive links between methane production and a high amount of fatty acids.ConclusionsOur results suggest that heterologous WRI1 expression in transgenic plants can be used to improve the metabolic potential for bioenergy purposes, in particular methane production.

【 授权许可】

CC BY   
© The Author(s) 2023

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